Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UNIPROT:P01185 (vasopressin)
 23,126 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

This study was designed to determine if vasopressin stimulates the formation of thromboxane B2 in freshly isolated hamster hepatocytes and if so, whether this thromboxane synthesis plays a role in the vasopressin-induced efflux of 45Ca++ from these cells. Thromboxane synthesis was found to be linear for 15 minutes, to be stimulated by arachidonic acid, and to be inhibited by indomethacin and by 7-(1-imidazolyl) heptanoic acid (7IHA), a selective inhibitor of thromboxane synthetase. Vasopressin stimulation of thromboxane synthesis was dose-related. Pretreatment with 7IHA and meclofenamate significantly increased basal 45Ca++ uptake by the cells. Neither 7IHA nor meclofenamate inhibited the ability of vasopressin to reduce the 45Ca++ content of the isolated hepatocytes. These results indicate that vasopressin stimulates thromboxane synthesis in hepatocytes but suggest that thromboxane does not play a role in the vasopressin-induced reduction in 45Ca++ content in this cell type.
 ...
PMID:Vasopressin stimulates thromboxane synthesis in isolated hamster hepatocytes: relation to hepatocyte calcium content. 641 86

The time course of vasopressin stimulation of water flow and immunoreactive thromboxane B2 (iTXB2) and prostaglandin E (iPGE) biosynthesis was studied in the isolated toad urinary bladder. Vasopressin (25 mU/ml) significantly stimulated iTXB2 synthesis within 8 min, synthesis reaching a maximum rate by 17 min. iPGE synthesis was significantly stimulated within 8 min, remaining unchanged for 24 min. Maximum vasopressin-stimulated water flow was reached between 16 and 24 min. 7-(1-Imidazolyl)-heptanoic acid (7IHA), a thromboxane synthetase inhibitor, inhibited both vasopressin-stimulated water flow and iTXB2 synthesis in a dose-dependent fashion, but did not affect iPGE synthesis. Vasopressin-stimulated water flow and iTXB2 synthesis were significantly correlated (r = 0.75, n = 24, P less than 0.001). 13-Azaprostanoic acid (13APA), a thromboxane antagonist, inhibited vasopressin-stimulated water flow in a dose-dependent fashion. Inhibition of arachidonic acid metabolism abolished the effects of 7IHA and 13APA on vasopressin-stimulated water flow. 7IHA and 13APA had no effect on cAMP-stimulated water flow. These results confirm that vasopressin stimulates TXA2 and PGE synthesis and support the hypothesis that TXA2 is a positive modulator of vasopressin-stimulated water flow in the toad urinary bladder.
 ...
PMID:Vasopressin-stimulated water flow is decreased by thromboxane synthesis inhibition or antagonism. 677 24