Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P01185 (vasopressin)
 23,126 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

It has been previously demonstrated with freeze-fracture electron microscopy that vasopressin induces specific structural alterations of the luminal membrane of granular cells from toad urinary bladder in a dose-dependent fashion. These alterations consist of aggregated intramembranous particles and are observed both in the presence and absence of an osmotic gradient. We examined the effect of methohexital, a selective inhibitor of vasopressin-stimulated water flow, and the effect of phloretin, a selective inhibitor of urea permeability, on the structure of the granular cell luminal membrane. Methohexital treatment of the vasopressin-stimulated toad bladder reduced both the osmotic water flow and vasopressin-induced alterations of membrane structure to the same extent. Phloretin reduced urea permeability but not water flow or particle aggregation. Since neither agent affects vasopressin-stimulated sodium movement, these findings indicate that the phenomenon of particle aggregation is specifically related to vasopressin-induced water permeability and not to changes in urea or sodium permeability.
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PMID:Relationship of aggregated intramembranous particles to water permeability in vasopressin-treated toad urinary bladder. 40 87

1. Net water flow J(w), was measured across the abdominal skin of the toad Bufo marinus with a volumetric, automatic technique that allows for averaging J(w) over time intervals as short as 1 sec.2. Basal J(w) was very stable and corresponded to a coefficient of osmotic flow, L(PD), of ca. 15 x 10(-7) cm sec(-1) atm(-1) (or to an osmotic water permeability coefficient, P(f), of 20 mum sec(-1)).3. Both vasopressin and the beta-adrenergic agonist, isoprenaline, triggered high hydrosmotic responses that could lead to P(f) values exceeding 250 mum sec(-1). The effect of isoprenaline was very reproducible while that of vasopressin varied considerably.4. Methohexital and propranolol selectively inhibited the hydrosmotic effects of vasopressin and isoprenaline, respectively, whereas amiloride and ouabain had no effect.5. Mutual inhibition was found between vasopressin and isoprenaline in skins very sensitive to vasopressin. In less sensitive skins isoprenaline further increased J(w) despite exposure of the epithelia to supramaximal concentrations of vasopressin.6. Differential reactivity to vasopressin was found between the skin and the bladder taken from the same toad. In some instances, the bladder responded normally to vasopressin while the skin was totally unresponsive, suggesting the presence of osmoregulatory mechanisms exerting a local modulation of the vasopressin action in different target epithelia of the same animal.
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PMID:Osmotic water flow across the abdominal skin of the toad bufo marinus: effect of vasopressin and isoprenaline. 681 22