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Target Concepts:
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Query: UNIPROT:P01185 (
vasopressin
)
23,126
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Short-term activation of microsomal
cholesterol ester hydrolase
by glucagon, cAMP analogues, and
vasopressin
in isolated rat hepatocytes is described. Glucagon led to a dose- and time-dependent activation of cholesteryl oleate hydrolysis, but values returned to basal levels within 120 min. Exposure of isolated hepatocytes to 0.5 mM concentrations of dibutyryl-cAMP or 8-[4-chlorophenylthio]-cAMP, or 25 microM forskolin caused persistent activation of
cholesterol ester hydrolase
activity after a lag period of 30 min. The three agents resulted in early marked intracellular accumulation of cAMP that declined progressively, and moderate and sustained reductions in the diacylglycerol content. The actions of glucagon on hepatocytes were inhibited by pretreatment of cells with 10 nM [8-arginine]
vasopressin
. Vasopressin elicited a consistent and sustained increase in
cholesterol ester hydrolase
activity and diacylglycerol without affecting cAMP while reducing the effect of glucagon on cAMP. Furthermore, the effects of glucagon and
vasopressin
on the activation of
cholesterol ester hydrolase
were not additive despite the similarity of their stimulation of diacylglycerol formation. Blockade of
vasopressin
-mediated activation of
cholesterol ester hydrolase
and diacylglycerol content were induced by excess prazosin. These data suggest that stimulation of microsomal
cholesterol ester hydrolase
in isolated liver cells may involve at least two signal transduction systems.
...
PMID:Stimulation of microsomal cholesterol ester hydrolase by glucagon, cyclic AMP analogues, and vasopressin in isolated rat hepatocytes. 890 Apr 56
