Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P01185 (
vasopressin
)
23,126
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
A synthetic oligodeoxynucleotide encoding the
vasopressin
peptide was ligated to the 3' terminal codon of sacB, the structural gene of
levansucrase
. This gene fusion was integrated into the chromosome of a Bacillus subtilis strain able to overproduce
levansucrase
. The extracellular production of the hybrid protein, consisting of the whole
levansucrase
primary sequence plus the nine amino acids of the
vasopressin
peptide added at the C-terminal end, represented 50-55% of that found for the wild-type
levansucrase
(20 mg l-1). The purified hybrid protein displayed the same conformational stability, protease insensitivity and enzymic properties as the wild-type
levansucrase
. However, the rate and the yield of the unfolding-folding transition at the pH and temperature used for bacterial growth were lower in the case of the hybrid protein; the latter also required a higher iron concentration to be completely folded.
...
PMID:Peptide carrier potentiality of Bacillus subtilis levansucrase. 152 90
