Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P01185 (
vasopressin
)
23,126
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
1. Rabbit antisera against
arginine-vasopressin
(
AVP
) were evaluated for sensitivity and specificity in a radioimmunoassay based on the extraction of
AVP
from plasma and urine on to
Florisil
. 2. Comparison of the immunoreactivity of
AVP
with analogues showed that one antiserum (R2) reacted principally with the hexapeptide ring and another (R4) bound to the tripeptide tail and was reactive with some reduction and hydrolytic products of the native peptide. 3. The minimum amount of
AVP
measurable in the radioimmunoassay was 1 pg. The extraction of
AVP
from plasma and urine gave a recovery of 93 per cent (sd 5 per cent). A plasma sample repeatedly assayed with R2 gave a value of 1.4 ng/1 (sd 0.2, n=12). 4. The antiserum specific for the hexapeptide ring (R2) showed that in normal subjects
AVP
concentration ranged from 3.2 +/- 2.52 ng/1 after dehydration to 0.16 +/- 0.1 ng/1 after water loading.
...
PMID:The specificity of antisera for the radioimmunoassay of arginine-vasopressin in human plasma and urine during water loading and dehydration. 107 Apr 18
