Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P01185 (vasopressin)
 23,126 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

To define how renal papillary epithelial cells respond to wide changes in the ionic and osmotic composition of their environment, measurements were made of the transmembrane potential differences (PD) of rat renal papillary epithelial cells in vitro in media containing 100 mM NaCl, 100 mM KCl, 1.5 mM CaCl2, and 1 mM MgSO4 plus varying amounts of urea and/or sucrose up to 1,400 mM. Glass microelectrodes (resistance 25-75 M Omega) were used. With added sucrose, no change in PD from the initial value of -9.3 +/- 1.3 (SD) mV (n = 29) (cell interior negative) was found. With added urea, alone or while osmolality was maintained nearly constant with sucrose, the PD changed in a triphasic manner, depolarizing to -5.3 mV at 50 mM urea, hyperpolarizing to -20.0 mV at 150 mM urea, and then depolarizing again to -5.5 mV at 1,400 mM urea. When bath potassium was decreased to 10 meQ/liter (choline replacement) the PD hyperpolarized to -46.9 +/- 5.0 (SD) mV (n = 32). DDAVP, a nonpressor vasopressin analogue, and 8-bromo-cAMP depolarized the membrane to -5 mV in the presence of urea but did not change PD when urea was absent. These observations suggest an interaction between urea and ionic movement or conductance in rat renal papillary epithelial cells.
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PMID:Transmembrane potential of renal papillary epithelial cells: effect of urea and DDAVP. 400 53

The role of synaptic input to the vasopressin neurons in hypertonicity-induced increase in vasopressin mRNA content was evaluated. Synaptic connection with the anterior hypothalamus is required for hypertonicity to increase vasopressin release. However, the potential for other mechanisms to induce the increase in vasopressin mRNA content is suggested by the fact that hypertonicity induces depolarization of supraoptic neurons independently of synaptic input. Explants of the hypothalamoneurohypophysial system were used to study the effect of depolarization and hypertonicity in the presence and absence of nonspecific synaptic blockade by 15 mM MgSO4 or blockade of excitatory amino acid receptors with kynurenic acid. Vasopressin release and mRNA content were increased by depolarization with 40 mM KCl and by exposure to hypertonicity (P < 0.05). Basal and osmotically stimulated vasopressin release was decreased by MgSO4 and by kynurenic acid. Both agents prevented the hypertonicity-induced increase in vasopressin mRNA content. Thus either synaptic input or increased VP release is required for hypertonicity to increase vasopressin mRNA, and excitatory amino acids are implicated in this response.
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PMID:Osmotic stimulation of vasopressin mRNA content in the supraoptic nucleus requires synaptic activation. 773 86