Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P01178 (oxytocin)
 15,767 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The isolated urinary bladder of the toad responds to neurohypophyseal hormone with a net increase of water transport from the mucosal to the serosal solution in the presence of an osmotic gradient. This response is mediated intracellularly by cyclic 3',5'-adenosine monophosphate (AMP). The present study demonstrates that hydroosmotically active substances such as oxytocin, dibutyryl cyclic 3',5'-AMP, and theophylline, but not hydroosmotically inactive substances, induce the uptake of horseradish peroxidase from the mucosal solution. Peroxidase taken up by the mucosal cells is demonstrable in small tubules and vesicles, and eventually accumulates in lysosomes. The uptake of peroxidase from the serosal solution into similar bodies in the mucosal cells is not hormone-dependent. It is also shown that peroxidase does not penetrate the tight junction from either the mucosal or serosal solution. These results extend previous findings which implicated the apical membrane of the mucosal epithelium as the site affected by neurohypophyseal hormones. A mechanism based on secretory phenomena is proposed as a framework for future investigations of apical membrane permeability changes and pinocytosis.
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PMID:Correlation between pinocytosis and hydroosmosis induced by neurohypophyseal hormones and mediated by adenosine 3',5'-cyclic monophosphate. 432 55

The comparative distribution of nine peptides was examined in the L4 segment of the rat cord using the peroxidase antiperoxidase technique. The peptides examined were substance P, neurotensin, cholecystokinin, methionine-enkephalin, oxytocin, neurophysin, adrenocorticotrophin, thyrotropin releasing hormone, and vasoactive intestinal polypeptide. No transport blocking agents were used and in spite of this cell bodies containing substance P, neurotensin, cholecystokinin, and methionine-enkephalin were observed. All peptides except for thyrotropin releasing hormone were observed in fibers in laminae I and II. All peptides were present in the area around the central canal, lamina X. Each peptide had its own characteristic distribution within fibers in the gray and white matter.
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PMID:The distribution of nine peptides in rat spinal cord with special emphasis on the substantia gelatinosa and on the area around the central canal (lamina X). 616 70

The axonal endoplasmic reticulum (ER) and synaptic-like (micro)vesicles within axon terminals of the neurohypophysis and their contribution to the secretory process in hypothalamo-neurohypophysial neurons have been investigated cytochemically in normal mice and in mice given 2% salt water to drink for stimulation of hormone synthesis in and release from these neurons. Cytochemical techniques included the peroxidase-antiperoxidase (PAP) immunocytochemical method for localization of neurophysin, wheat germ agglutinin-horseradish peroxidase (WGA-HRP) as a tracer for the anterograde axonal transport of membrane from within the perikaryon, and blood-borne native horseradish peroxidase (HRP) as a tracer for internalized axon terminal membrane. The primary antiserum employed was directed against neurophysins I and II, the carrier proteins for the peptide hormones oxytocin and vasopressin, respectively. PAP reaction product was observed over neurosecretory granules but never over the endoplasmic reticulum, microvesicles or other organelles in axons and terminals of the neurohypophysis. WGA-HRP was delivered extracellularly to cell bodies of paraventricular neurons by cerebral ventriculocisternal perfusion. Internalized perikaryal surface membrane tagged with WGA-HRP was recycled through the innermost Golgi saccule (GERL) from which neurosecretory granules were formed. The anterograde axonal transport of membrane-bound WGA-HRP was manifested within the neurosecretory granules; WGA-HRP did not label the axonal reticulum or terminal microvesicles in the neurohypophysis. Blood-borne native HRP endocytosed into neurohypophysial terminals was associated with a plethora of microvesicles measuring 40-70 nm in diameter and vacuoles similar in size to the 100-300-nm-wide neurosecretory granules. The microvesicles contributed to the formation of numerous vacuoles. The internalization of axon terminal membrane as microvesicles incorporating HRP was quantitatively greater than vacuoles in both salt-stressed and control mice. The results suggest that in the hypothalamo-neurohypophysial system of the mouse the axonal ER and terminal microvesicles are not involved in the transport, storage, and exocytosis of neurosecretory material and perhaps other molecules processed through the innermost Golgi saccule. Nevertheless, a prominent population of the microvesicles within axon terminals of the neurohypophysis does participate in the secretory process. These vesicles are involved directly in the internalization of the terminal surface membrane subsequent to release of secretory granule content.(ABSTRACT TRUNCATED AT 400 WORDS)
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PMID:Further studies of the secretory process in hypothalamo-neurohypophysial neurons: an analysis using immunocytochemistry, wheat germ agglutinin-peroxidase, and native peroxidase. 620 13

Various cytophysiological aspects of the pars intermedia of the pituitary are discussed. Cells containing melanocyte-stimulating hormone (MSH) have been studied under normal and experimental conditions. They react to variations in ionic equilibrium, but without any clear correlation with natraemia and osmotic blood pressure. The MSH-cell stimulation in hypernatraemic mice, which is not inhibited by bromocriptine, seems more specific than the stimulation in hyponatraemic mice, which is blocked by bromocriptine. The existence of a corticotropic cell system has been clearly demonstrated in the mouse (where it is particularly obvious), in the rat and in the cat but its significance is not clear. Although very poorly vascularized, the pars intermedia is rapidly invaded by tracer protein (horseradish peroxidase) injected either intravenously or intracerebroventricularly. The hypophysial cleft rapidly stores the tracer which can be resorbed by macrophagic epithelial cells lying free in the colloid contained in the cleft. Horseradish peroxidase lingers in the pars intermedia but is rapidly eliminated from the other hypophysial lobes after intraventricular (third ventricle) injection. Diffuse innervation of the pars intermedia applies to both glandular (MSH and ACTH) and non-glandular (epithelial and stellate) cells. While aminergic innervation of the pars intermedia is obvious, cholinergic innervation has not been demonstrated ultrastructurally or histochemically. Peptidergic fibres only occasionally penetrate marginal areas of the pars intermedia and seldom establish synaptic contacts with glandular cells. A specific relationship might exist between the pars intermedia and oxytocin fibres in view of the marginal distribution of the latter in the neural lobe. Numerous stellate cells of the pars intermedia react intensely with antiserum to gliofibrillar acid protein, indicating their astrocyte nature, which reinforces the idea of an analogy between the folliculo-stellate system of the hypophysis and the glial cells.
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PMID:Fine structure and cytochemistry of the mammalian pars intermedia. 626 74

Horseradish peroxidase (HRP), injected into the rat caudal medulla oblongata, was detected by immunoperoxidase staining in 120 microns frozen sections, allowing examination of both the distribution and morphology of transporting neurons. In the hypothalamus, several groups of HRP-labeled neurons could be distinguished on the basis of location of the neurons, neural cell size and morphology of the neural processes. Most HRP-labeled neurons were found in the posterior half of the hypothalamus, although scattered single neurons were present as far rostral as the anterior hypothalamus and preoptic area. Prominent groups of HRP-labeled neurons were found in the paraventricular, dorsomedial and arcuate nuclei, near the fornix at two separate levels, and in the lateral posterior hypothalamus. Based on comparison with peptide immunohistochemistry it seems likely that many magnocellular oxytocin, vasopressin and neurophysin neurons in the paraventricular nucleus, and a few ACTH/beta-endorphin neurons in the arcuate nucleus may project to the caudal medulla oblongata.
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PMID:Hypothalamic neurons projecting to the rat caudal medulla oblongata, examined by immunoperoxidase staining of retrogradely transported horseradish peroxidase. 630

The magnocellular neurones of the supraoptic nucleus which synthesize and secrete vasopressin and oxytocin have been commonly regarded as simple "output" neurones in that they receive an input, generate an action potential and in turn release hormone from their terminals in the posterior pituitary. Three lines of evidence are presented which suggest that rat supraoptic nucleus neurons also have axon collaterals which terminate in the hypothalamus close to the nucleus. Small injections of horseradish peroxidase were made directly into the nucleus in hypothalamic slices, allowing visualization of the axons of supraoptic neurones. Collaterals of these axons could be observed in regions both dorsal and dorsolateral to the supraoptic nucleus. In a separate series of experiments, sections of perfusion-fixed hypothalamus were stained for vasopressin and oxytocin using specific antisera. Peptide-containing collaterals of both types were observed near the supraoptic nucleus, in a region similar to that seen after horseradish peroxidase injections. Finally, electrophysiological studies were carried out on hypothalamic slices containing the supraoptic nucleus. A small concentric bipolar stimulating electrode was placed directly into the nucleus and activity of lateral hypothalamic neurones within 0.1-1 mm of the nucleus was recorded. Of 68 neurones studied, 52 were excited by supraoptic stimulation via a synaptic pathway that could be blocked by Ca2+ -free solutions containing 18 mM Mg2+. These studies suggest that supraoptic neurones communicate via axon collaterals with other neurones in the lateral hypothalamus, in addition to their previously well characterised functional role in neurosecretion.
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PMID:Axon collaterals of supraoptic neurones: anatomical and electrophysiological evidence for their existence in the lateral hypothalamus. 632 27

Comparative ultrastructural localization of corticotropin-releasing factor (CRF) and oxytocin was performed in the rat median eminence of Long Evans and Brattleboro rats. The peroxidase-antiperoxidase technique used on serial ultrathin sections revealed CRF and oxytocin neurosecretory granule colocalization in the same fibers of the internal layer running towards the posterior pituitary. It is probable that both these peptides coexist in the same granules. In the Brattleboro rats, while genetically lacking vasopressin, CRF was nevertheless shown to be present. In these rats, as was demonstrated in the Long Evans rats, CRF distribution paralleled that of oxytocin only in the internal zone of the median eminence.
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PMID:Comparative immunoelectron microscopic localization of corticotropin-releasing factor (CRF-41) and oxytocin in the rat median eminence. 633 45

The distribution of vasopressin-, oxytocin- and LHRH-containing nerve fibers in the pineal organ of the dog was demonstrated by use of the peroxidase-antiperoxidase immunohistochemical technique. These neuropeptide-containing fibers penetrated through the pineal stalk from the brain, mainly from the posterior commissural region, into the pineal organ. The vasopressin fibers were the most prominent in number, oxytocin fibers and LHRH fibers were the least. Most of these fibers were found in the proximal part of the pineal organ, but some of them were also observed in the distal part. These peptidergic fibers were distributed not only in the perivascular spaces but among the parenchymal cells.
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PMID:Immunohistochemical studies on the peptidergic nerve fibers in the pineal organ of the dog. 635 38

The presence of neurophysin, oxytocin and vasopressin in the bovine corpus luteum was examined immunocytochemically. Tissue blocks of corpora lutea from pregnant and non-pregnant animals were fixed with glutaraldehyde/paraformaldehyde fixative and immunostained by the peroxidase-antiperoxidase (PAP) method. The simultaneous presence of immunoreactive oxytocin and immunoreactive oxytocin-neurophysin was demonstrated in large luteal cells of non-pregnant animals, while no staining for vasopressin or vasopressin-neurophysin was observed. None of the peptides were detected in the corpus luteum of pregnant animals. The small luteal cells were not found to be stainable at any time.
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PMID:Immunocytochemical evidence for the presence of oxytocin and neurophysin in the large cells of the bovine corpus luteum. 638 23

Neural elements immunoreactive for ovine corticotropin releasing factor-like immunoreactivity (oCRF-LI) were shown to be present in the diencephalon of the pigeon by using peroxidase-antiperoxidase immunocytochemistry. The external zone of the anterior median eminence contains a rich network of varicose oCRF-LI fibers in close proximity to the pituitary portal capillaries. Perikarya reactive for oCRF-LI were found in several regions known to innervate the median eminence and gave rise to axons which joined the hypothalamo-hypophyseal tract. A close topographical relationship between neurophysin-containing and oCRF-LI-containing neurons was found in anterior periventricular regions. These observations suggest that oCRF-LI material might be involved in the hypothalmic control of anterior pituitary hormone secretion in birds, and that neurophysin- and oCRF-LI-producing nerve cells might be functionally coupled.
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PMID:Comparative localization of neurons containing ovine corticotropin releasing factor (CRF)-like and neurophysin-like immunoreactivity in the diencephalon of the pigeon (Columba livia domestica). 638 81


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