Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P01178 (oxytocin)
 15,767 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

To determine the possible roles of tumor necrosis factor-alpha (TNFalpha) and nitric oxide (NO) in the bovine oviduct, ampulla and isthmus collected during the estrous cycle were exposed for 18 h to TNFalpha, NO donor (NONOate), NO synthase inhibitors (L-NOARG, L-NAME and AMT) and oxytocin (OT) as a positive control. Prostaglandins (PGs) and NO(2)/NO(3) in conditioned media were measured. TNFalpha stimulated PGF(2alpha) secretion on Day 0 (onset of estrus = Day 0) and Days 2-3, in both the ampulla and isthmus, but on Days 18-20 only in ampulla. TNFalpha increased PGE(2) secretion in both fragments in each phase. NONOate did not affect PGF(2alpha) secretion on Days 18-20, whereas this NO donor stimulated PGF(2alpha) secretion in both fragments on Day 0 and Days 2-3. TNFalpha increased NO(2)/NO(3) production in every examined phase in the ampulla and on Days 2-3 in the isthmus. L-NAME lowered NO(2)/NO(3) production regardless of phase or fragment. L-NOARG and AMT lowered NO(2)/NO(3) production in both fragments on Day 0 and Days 2-3. The possible role of TNFalpha, NO or PGs on the oviductal contractility during the early-luteal phase was also examined. Neither TNFalpha nor NONOate influenced contractility in either fragment. Although PGF(2alpha) stimulated the contraction in both fragments, PGE(2) decreased it. When taken together, TNFalpha seems to play some role as a modulator of PGF(2alpha) and PGE(2) production and for transferring the embryo from the oviduct to the uterus by stimulating NO production in the bovine oviduct.
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PMID:Effects of tumor necrosis factor-alpha and nitric oxide on prostaglandins secretion by the bovine oviduct differ in the isthmus and ampulla and depend on the phase of the estrous cycle. 1959 30