Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UNIPROT:P01178 (oxytocin)
 15,767 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The evaluation of a radioimmunoassay of oxytocin is described. The method involved careful collection and transportation of blood at 4 degrees C, acidification of the plasma, extraction with Fuller's earth and radioimmunoassay using antisera raised in rabbits immunized against oxytocin conjugated to bovine serum albumin and 125I-labelled oxytocin. The antisera showed insignificant cross-reaction with a variety of small peptides including vasopressin and vasotocin. The limit of detection of the assay was 2.5 pg with intra-assay and interassay coefficients of variation of 7-15% and 12-18% respectively. Seventy-seven per cent (88 out of 116) of the pregnant women tested had detectable maternal plasma oxytocin. Serial samples of maternal plasma showed a significant increase in oxytocin from the first to the second stage of labour and a significant decrease in the third stage. Oxytocin concentrations in the umbilical arterial plasma were significantly higher in patients in labour. The significance of these findings is discussed.
 ...
PMID:Radioimmunoassay of oxytocin. 62 20

Two thousand seven hundred ninety two contraction stress tests (CST) were analysed. Oxytocin was administered in 1730 of these to stimulate uterine contractions and the hyperstimulation which occurred in 48 tests (2.8%) was studied extensively. Seven patients went into labor within 24 hours of the hyperstimulation. Two infants weighed less than 2500 g. One was mature but small-for-dates and the other (who died neonatally) had a trisomy-18 syndrome with severe congenital abnormalities. There were no other intrapartum or neonatal complications. Ten CSTs demonstrated hypertonicity without any preceding oxytocin administration. No harmful effects from the hyperstimulation could be found.
 ...
PMID:Hyperstimulation of the uterus during the oxytocin stress test. 62 47

A significant elevation in plasma prolactin was observed 10 min following the intravenous injection of 100 microgram of melatonin into either estrogen-progesterone (EP) primed or into nonsteroid-treated male rats. 60 min postinjection in the EP primed rat, the groups treated with 100 microgram or 10 mg of melatonin had signficantly elevated plasma prolactin levels while no effect was observed with these same doses in the nonsteroid-treated rats. Compared to diluent-treated controls, a significant elevation in plasma prolactin was observed at 10, 20 and 60 min following the intravenous injection of either 1 microgram arginine vasotocin (AVT) or 1 mg melatonin into EP primed male rats. A consistent rise in plasma prolactin was also evident after the injection of 1 microgram of either arginine vasopressin, lysine vasopressin or AVT. Oxytocin had no effect on plasma prolactin values. The intravenous administration of 1 microgram of (deamino-1,6 dicarba, 8-arginine)-vasotocin caused a significant elevation of plasma prolactin 10 and 20 min after injection. However, the injection of another analogue of AVT, (4-leucine, 8-arginine)-vasotocin, had no effect on prolactin release at the time points measured.
 ...
PMID:Effects of melatonin and natural and synthetic analogues of arginine vasotocin on plasma prolactin levels in adult male rats. 66 73

Fetal oxytocin contribution to the mother during spontaneous labor was investigated using a specific and sensitive radioimmunoassay to measure oxytocin in blood, amniotic fluid, and urine. In 26 subjects with spontaneous labor and vaginal delivery (Group I) and 18 subjects with cesarean section after labor (Group II), umbilical arterial plasma (UA) oxytocin concentrations were significantly higher than umbilical venous plasma (UV) ocytocin concentrations. With elective cesarean section (Group III), UA oxytocin concentration was 29.8 +/- 7.5 pg/ml and UV oxytocin concentration was 16.1 +/- 5.9 pg/ml (n = 14). In contrast, the mean UV oxytocin concentration was higher than the mean UA oxytocin concentration, when oxytocin was given to the mothen concentration in Groups I and II was significantly higher than in Group III. Amniotic fluid oxytocin concentrations in Group I and II patients were higher than in Group III. Oxytocin was also present in fetal urine. The findings indicate that during spontaneous labor, oxytocin is produced by the fetus and flows toward the maternal circulation.
 ...
PMID:Fetal contribution to oxytocin in human labor. 68 60

The perinatal prognostic significance of a type of Prepathological Oxytocin Stress Test, namely Transient Bradycardia coincident with a period of hyperdynamia in otherwise normal test, is analyzed. The 43 studied cases are compared to a group of 492 patients responding normally. The significantly higher incidence of intrapartum meconium and fetal distress, low Apgar Score and neurologically affected newborns seems to demonstrate that the Prepathological Test is a real entity. The possible fetal risk and the usefulness of the Oxytocin Test are discussed.
 ...
PMID:Significance of the transient bradycardic pattern in prepathological oxytocin stress test. 70 23

We carried out the Oxytocin Challenge Test (or OCT as American authors call it) by injecting 5 m.U. per minute into pregnant women who are suspect of having high fetal risk from the 34th week of pregnancy onwards. The material that we have examined is from 1,366 cases with 1,827 tests. The perinatal mortality over all was 13.17 per thousand of the cases. We have described three types of response: normal (87.44%), pre-pathological (7.77%) and pathological (6.78%). We have concluded that the test has a good prognostic value and point out the significance of the pre-pathological results, which we think have up till now not been accorded sufficient attention.
 ...
PMID:[The antepartum oxytocin fetal assessment challenge test 1,827 case histories. Significance of "prepathological testing". "Results" (author's transl)]. 71 44

The influence of 500 microunits oxytocin as well as 50 micrunits lysine-vasopressin on hypothalamic self-stimulation rate after intraventricular application was studied. Oxytocin caused an increase and vasopressin a decrease in the self-stimulation rate.
 ...
PMID:[Effect of the intraventricular administration of vasopressin and oxytocin on hypothalamic self stimulation]. 74 65

Four cases of water intoxication in connection with oxytocin administration during saline-induced abortions are described. The mechanism of water intoxication is discussed in regard to these cases. Oxytocin administration during midtrimester-induced abortions is advocated only if it can be carried out under careful observations of an alert nursing staff, aware of the symptoms of water intoxication and instructed to watch the diuresis and report such early signs of the syndrome as asthenia, muscular irritability, or headaches. The oxytocin should be given only in Ringers lactate or, alternately, in Ringers lactate and a 5 per cent dextrose and water solutions. The urinary output should be monitored and the oxytocin administration discontinued and the serum electrolytes checked if the urinary output decreases. The oxytocin should not be administered in excess of 36 hours. If the patient has not aborted by then the oxytocin should be discontinued for 10 to 12 hours in order to perform electrolyte determinations and correct any electrolyte imbalance.
 ...
PMID:Water intoxication associated with oxytocin administration during saline-induced abortion. 80 83

Plasma levels of prolactin and TSH were determined by radioimmunoassay in urethaneanaesthetized lactating rats during suckling. Oxytocin release was monitored by recording intramammary pressure. Application of ten pups, 3 h after administration of urethane (1-1 g/kg, i.p.), evoked a parallel rise in prolactin and TSH concentrations which reached a maximum during the 3rd hour of suckling and then declined. Peak hormone concentrations represented a 25-fold increase in prolactin and a ten-fold increase in TSH. Suckling also elicited a pulsatile (every 5-10 min) release of 0-5--1-0 mu. oxytocin. The gradual rise in prolactin and TSH occurred between the 1st and 20th oxytocin pulses. Intravenous injection of thyrotrophin-releasing hormone (TRH) into unsuckled, anaesthetized lactating rats resulted in a 7- to 30-fold increase in TSH concentration, whereas prolactin levels showed no substantial change. These results indicate that suckling releases TSH as well as prolactin in the urethaneanaesthetized rat. However, the absence of prolactin release after injections of TRH makes it unlikely that both endocrine responses are regulated solely by the actions of this one releasing hormone.
 ...
PMID:Plasma concentrations of prolactin and thyrotrophin during suckling in urethane-anaesthetized rats. 82 93

Oxytocin analogues which combine high oxytocic activities with negligible antidiuretic and pressor activities have been studied. [4-Threonine,7-glycine]oxytocin, [1-(L-2-hydroxy-3-mercaptopropionic acid),4-threonine,7-glycine]oxytocin, and [1-(L-2-hydroxy-3-mercaptopropionic acid)]oxytocin were found to possess the following specific biological activities respectively: rat uterotonic, 270 +/- 10, 337 +/- 23, 1542 +/- 0.4; rat antidiuretic, 0.002 +/- 0.0008, 0.048 +/- 0.005, 40.3 +/- 2.4. The results are analyzed from a conformation-activity viewpoint in a continued attempt to evaluate the scope and limitations of this approach in comparison to structure-activity studies.
 ...
PMID:Combined high oxytocic with negligible antidiuretic and pressor activities in multisubstituted oxytocins. 85 Feb 33


<< Previous 1 2 3 4 5 6 7 8 9 10 Next >>