Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P01178 (oxytocin)
 15,767 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The diagnosis of pregnancy described in this article is based on the observation of milk ejection which, in the case of corpus luteum maintenance, results from the release of luteal oxytocin induced by intravenous administration of a non luteolytic dose of PGF2 alpha. The tests were performed in 410 lactating cows, 18-22 days after insemination (mean 19.95 days, SD 0.75), 3 h prior to the evening milking. A cannula was placed in the left fore-teat; when the cisternal milk flow ceased, a small dose of a prostaglandin F2 alpha analogue (256 micrograms Dinolytic) was injected. If a corpus luteum was present, the alveolar milk flow (1,276 +/- 570 ml) started 86 +/- 35 s later and pregnancy could be presumed. If the corpus luteum was no longer functional, the milk flow did not start again and the cow was considered not pregnant. The accuracy of positive results, ie 72.3%, significantly exceeded that obtained by radioimmunoassay of plasma progesterone used as a reference method, ie 68.6% (P less than 0.05). By contrast, the accuracy of negative results was lower, ie 93.5 vs 100%, (P less than 0.05): of the 195 pregnant cows which proceeded to term, 10 did not respond to the milk ejection test. In spite of these reserves, the test has the advantage of being inexpensive, rapid (about 5 min) and easy to interpret, ie either the milk flows or it does not. In addition, it may be used early, because from day 20 after Al the reliability of prediction of non pregnant cows (67.0%) was significantly higher when compared to the use of progesterone radioimmunoassay (58.6%) (P less than 0.05). With such a test, non pregnant cows can be reinseminated immediately and not only after a 3-week period. This may contribute to reducing the length of the infertile period.
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PMID:[Early diagnosis of pregnancy in the cow as assessed by milk ejection induced by luteal oxytocin]. 138 95

Seven lactating Lacaune ewes underwent either a total luteectomy on day 19 of pregnancy (D19) (compensated from that stage by a daily progesterone supplementation of 25 mg to ensure embryonic survival; group 1:4 animals) or a control laparotomy (group 2: 3 animals). Intra-jugular injection of 200 micrograms of a synthetic PGF2 alpha analogue (Dinolytic, Upjohn) caused an increase in the intramammary pressure (IMP) and a concomitant rise in oxytocinaemia only in the presence of a corpus luteum, ie in all ewes of groups 1 and 2 before D19 and only in those of group 2 after that stage. These experiments confirm that the corpus luteum, and not the other ovarian compartments, releases oxytocin when prostaglandin F2 alpha is administered.
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PMID:[Suppression by luteectomy of the simultaneous increase in intramammary pressure and blood oxytocin levels induced by the injection of PGF2-alpha in ewes]. 233 9

Milk ejection after intrajugular injection of 256 micrograms of a synthetic PGF2 alpha analogue (Dinolytic, Upjohn) was measured every 2 days until D60 in 7 pregnant cows and until D25 in 5 non pregnant inseminated cows (trial 1). There was a very close correlation (r around + 0.98) between the values of the 3 parameters used to characterize milk ejection, i.e. amplitude of intramammary pressure deflection, surface area under the pressure curve, alveolar milk volume collected by a teat probe. In the two groups of animals, these values behaved similarly until D15 with a sudden increase between D2 and D7. From D15 they decreased abruptly in females where insemination had failed while they still remained at a high level until D25 (then decreasing until D60) in those presumed to be pregnant (fig. 2). These responses were assumed to result from the release of luteal oxytocin and it is suggested that a single injection of 256 micrograms of Dinolytic on D20, together with milk ejection control using a teat probe, would be a rapid, little expensive method for the farmer (without risks of luteolysis) (trial 2 and fig. 3) to distinguish between pregnant and non pregnant cows.
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PMID:[Milk ejection induced by PGF2 alpha during the first two months following insemination of cows. Application to pregnancy diagnosis]. 324 95

The intrajugular administration of 200 micrograms of Dinolytic (tromethamine salt of PGF2 alpha) to lactating ewes caused an intramammary pressure (IMP) increase only during the luteal phase of the sexual cycle (group A). The increase in progesteronemia, induced by three daily injections of 2.5 micrograms of LH-RH, did not modify response amplitude (group B). On the other hand, bilateral ovariectomy (groupe C) led to the suppression of those responses, but supplementary oestroprogestative (table 1) did not re-establish them. It is probable that the milk ejection caused by PGF2 alpha resulted from a release of oxytocin by the corpus luteum. The ineffectiveness of the exogenous and endogenous reinforcement of progesterone therefore suggests that this hormone plays no part in the putative control of hypothalamic receptivity to prostaglandins.
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PMID:[Effects of PGF2 alpha prostaglandins on milk ejection in the ewe. Consequences of ovariectomy with or without estroprogestative complementation]. 353 79

This trial was conducted in a farrow-to-finish pig unit from November 1999 to February 2000. Since November 1998 an induction-of-parturition program was applied in gilts and sows with PGF2alpha (2 mL Dinolytic, i.m.) 113 d post service, followed by oxytocin (1 mL Intertocine-S, i.m.) 24 h later. This program resulted in a high proportion of animals farrowing within the working hours of the day. At mid December 1999 splay-legs and edematous swelling and reddening of the vulva started to be observed in newborn piglets. A concurrent decline of parameters related to parturition also was noticed. Mycotoxicological analyses of the feeds revealed a co-occurring contamination with deoxynivalenol and zearalenone. For a 4-week period, sows were divided into two groups: (a) an induction-of-parturition and (b) a non-induction-of-parturition group. Significant differences were found between the two groups relating to prevalence of dystocia (<.05) and pregnancy duration (<.05). Moreover, it was found that prevalence of splay-legs and swelling of the vulva were highly correlated (<.05) with reduction of percentage of sows farrowing within the working day and increase of pre-weaning mortality. It was concluded that such an induction-of-parturition program should be avoided during a Fusarium mycotoxicosis.
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PMID:Association of Fusarium mycotoxicosis with failure in applying an induction of parturition program with PGF2alpha and oxytocin in sows. 1139 24