Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P01178 (
oxytocin
)
15,767
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Oxytocin
(
OXT
) is a peptide hormone that plays a central role in the regulation of parturition and lactation.
OXT
signaling is mediated by
OXT
receptor (OXTR), which shows species- and tissue-specific expressions and gene regulation. In the present study, we examined the synthesis of
OXT
and OXTR in human placenta tissue according to gestational age. A total of 48 placentas were divided into early preterm, late preterm and term groups depending on gestational age, and expression of
OXT
and OXTR was evaluated. First,
OXT
and OXTR mRNA and protein were detected in normal placenta tissue via Q-PCR, Dot-blot and Western blot assay. Both
OXT
and OXTR levels in normal placenta increased gradually in the late stage of pregnancy, suggesting that local
OXT
may play a critical role in the function of the placenta. To determine the regulatory mechanism of
OXT
, placental BeWo cells were administrated estrogen (E2) or progesterone (P
4
), and expression of
OXT
and OXTR was tested. The mRNA and protein levels of
OXT
and OXTR were upregulated by E2 but blocked by co-treatment with P
4
In order to confirm the estrogen receptor (ESR)-mediated signaling, we administrated ESR antagonists together with E2 to BeWo cells. As a result, both
OXT
and OXTR were significantly altered by ESR1 antagonist (
MPP
) while moderately regulated by ESR2 antagonist (PHTPP). These results suggest that
OXT
and OXTR are controlled mainly by E2 in the placenta via ESR1 and thus may play physiological functions in the human placenta during the late stage of pregnancy.
...
PMID:The regulation of oxytocin and oxytocin receptor in human placenta according to gestational age. 2869
