Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P01178 (oxytocin)
 15,767 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A microcellular dispersion procedure for the rat neurohypophysis was developed, comprising tissue softening and dissociation using a special sieving sytringe. In preparatory studies the influence of mesh width, and treatment with trypsin, pronase or collagenase-hyaluronidase was investigated using light and electron microscopy, as well as with microchemistry by means of protein and lactate dehydrogenase activity determinations. Trypsinization gave the best results. In the final adopted procedure, 3 incubated neurohypophyses were sequentially sieved through a 200- and a 50-mum mesh. The resulting 50-mul dispersion was found to contain numerous ultrastructurally well-preserved pinched-off axonal endings (neurosecretosomes), and pituicytes often revealing processes. On the basis of DNA and oxytocin assays 11% of the pituicytes and 28% of the axonal cytoplasm were recovered. Oxytocin immunofluorescence microscopy showed hormone within the neurosecretosomes, but often also in the cytoplasm of pituicytes. Microdensity gradient centrifugation was performed on neurohypophyseal disperions, in order to obtain fractions enriched for neurosecretosomes and pituicytes. Fractions were characterized by means of phase contrast, oxytocin immunofluorescence and electron microscopy, as well as by oxytocin and DNA assays as respective markers. With a 10:14:22% (w/v) Ficoll gradient, fractions were obtained for which the relative purification was by a factor of 4 on the basis of DNA/oxytocin ratios.
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PMID:Enzymic preparation of neurosecretosome- and pituicyte-enriched fractions from the rat neurohypophysis. 18 63

The clinical course of labor in 53 cases with cervical injection of hyaluronidase (HD) and 51 cases with intravenous infusion of oxytocin to ripen the cervix was studied. The success rate of HD group (92.5%) was significantly higher than that of control group (62.8%). HD was without adverse side effects, and did not induce uterine contraction and therefore was preferable for high risk pregnancy cases. This method needed less time for cervical priming than others. The drug is easily available, not expensive and the method is simple. The animal experiments showed the mechanism of action of cervical ripening by HD was probably as follows: (1) the dissociation of ground substance in cervical connective tissue; (2) The destruction of blood vessels permit the migrating of leucocytes to surrounding tissue and resolve collagen; (3) stimulation of cervical fibroblasts to release prostaglandin.
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PMID:[Clinical study on uterine cervix ripening with hyaluronidase and animal experimentation on mechanism of action]. 811 27

Contractions of the rat uterus in response to trypsin, kallikrein, bradykinin, angiotensin II, oxytocin and acetylcholine, were abolished when an inside-out preparation was used. Sensitivity to Ba++, however, was preserved. In preparations in which the endometrium was mechanically removed, all above cited agonists elicited contractions. By treating the uterus with both collagenase and hyaluronidase, acetylcholine was able to induce a contraction when applied to the endometrium side of the uterus. The results show that a barrier for protease, peptides and acetylcholine is present in the mucosa of the rat uterus.
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PMID:Pharmacological demonstration of a barrier for protease, peptides and acetylcholine in the endometrium of the rat. 818 17