Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P01178 (oxytocin)
 15,767 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Changes in prolactin (PRL)-like bioactivity in rat milk after a longer isolation of litters and the effects of oxytocin (OT) were determined by Nb2 lymphoma cell bioassay and compared with the immunoreactivity using an enzyme immunoassay. First, we confirmed the appropriate dilution rates of rat milk which could neutralize the influence of an antimitogenic factor(s) in milk to Nb2 lymphoma cell proliferation. To prolong the litter removal, i.e. 6 h to 20 h, resulted in the increase in an antimitogenic factor(s) for Nb2 lymphoma cells in milk and in the significant decrease in milk PRL concentration measured by both assays, and plasma bioactive PRL level increased. The bioactivity/immunoreactivity ratio of PRL in milk accumulated in the mammary gland was 0.65 in rats after a 20-h isolation, but 1.18 after a 6-h isolation. The milk PRL concentration in rats isolated for 6 h decreased significantly within 45 min after the administration of OT and the plasma PRL concentration increased only slightly, but OT had no effect in rats after isolation for 20 h. The present findings indicate that milk PRL might transfer to plasma with the excessive engorgement of milk in the mammary gland and with OT administration. Moreover, PRL trapped in milk for a long period loses its biological activity more rapidly than its immunological activity, since bioactivity/immunoreactivity ratio in the 20-h isolation group was significantly lower than in the 6-h isolation group.
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PMID:Changes in prolactin bioactivity of milk following isolation from litter and oxytocin administration in rats. 907 19

The influence of exogenous oxytocin (OT) on maternal and infant behavior over 60 min of suckling, which followed 6 h of isolation, was investigated on Day 12 of lactation in rats. Mothers administered 1 IU of OT or saline through an indwelling atrial catheter and their litters indicated a similar nursing and suckling pattern, which was estimated by the crouching time of the mothers and the number of stretch reactions performed by the litters during a suckling period. To assess the alteration of the suckling intensity by OT administration, the plasma prolactin (PRL) level was determined by an Nb2 lymphoma cell bioassay. In the control group, the plasma PRL level increased and reached a peak at 45 min after the onset of suckling in 60% of the animals. The suckling-induced PRL release was completely inhibited and/or markedly delayed by OT administration. The difference in body weight of the litters before and after a suckling period was estimated as an index of the amount of milk suckled by the litters. There was no difference in the amount of milk between the control and OT-treated groups during a 60-min suckling period. However, it was significantly greater in the OT-treated group during the first 20 min of the suckling period. These results indicate that a dose of OT is a factor in the attenuation of the intensity of suckling done by the pups, whereas the nursing and suckling behavior is not influenced by OT administration.
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PMID:Exogenous oxytocin attenuates suckling-induced prolactin release but not maternal or infant behavior in lactating rats. 961 20

Neurohypophysial oxytocin (OT) and vasopressin (VP) genes are transcribed in thymic epithelium, while immature T lymphocytes express functional neurohypophysial receptors. Neurohypophysial receptors belong to the G protein-linked seven-transmembrane receptor superfamily and are encoded by four distinct genes, OTR, V1R, V2R and V3R. The objective of this study was to identify the nature of neurohypophysial receptor in thymic T cell subsets purified by immunomagnetic selection, as well as in murine thymic lymphoma cell lines RL12-NP and BW5147. OTR is transcribed in all thymic T cell subsets and T cell lines, while V3R transcription is restricted to CD4+CD8+ and CD8+ thymic cells. Neither V1R nor V2R transcripts are detected in any kind of T cells. The OTR protein was identified by immunocytochemistry on thymocytes freshly isolated from C57BL/6 mice. In murine fetal thymic organ cultures, a specific OTR antagonist does not modify the percentage of T cell subsets, but increases late T cell apoptosis further evidencing the involvement of OT/OTR signaling in the control of T cell proliferation and survival. According to these data, OTR and V3R are differentially expressed during T cell ontogeny. Moreover, the restriction of OTR transcription to T cell lines derived from thymic lymphomas may be important in the context of T cell leukemia pathogenesis and treatment.
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PMID:Neurohypophysial receptor gene expression by thymic T cell subsets and thymic T cell lymphoma cell lines. 1515 11