Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P01178 (oxytocin)
 15,767 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

An experiment was conducted to (i) determine whether administration of recombinant bovine interferon-alpha I1 (rBoIFN-alpha) attenuates oxytocin-induced release of prostaglandin F-2 alpha and (ii) confirm previous observations that rBoIFN-alpha causes acute changes in body temperature and circulating concentrations of progesterone. Cows were treated twice a day from Day 14 to Day 17 after oestrus with a control regimen (bovine serum albumin (BSA), i.m. + BSA intrauterine (i.u.)), rBoIFN-alpha, i.u. + BSA, i.m. (rBoIFN-IU) or rBoIFN-alpha, i.m. + BSA, i.u. (rBoIFN-IM). On Day 17, plasma concentrations of 13,14-dihydro,15-keto-prostaglandin F-2 alpha (PGFM) were measured after injection of oxytocin. Cows treated with rBoIFN-IU and rBoIFN-IM had longer oestrous cycles and luteal lifespans than control cows. A hyperthermic response and decline in plasma concentrations of progesterone was noticed after administration of rBoIFN-alpha on Day 14. On other days, the hyperthermic response was not present and the decline in progesterone was less pronounced. There was no significant effect of rBoIFN-alpha on circulating concentrations of oestradiol between Days 14 and 17. The release of PGFM induced by oxytocin was lower in cows treated with rBoIFN-alpha than in control cows. Oxytocin caused increased plasma concentrations of PGFM in four of five control cows, two of five rBoIFN-IU cows and two of five rBoIFN-IM cows. The peak PGF-2 alpha response to oxytocin (peak value after injection minus mean concentration before injection) was 257.8 +/- 61.3 pg/ml for control cows, 100.7 +/- 40.8 pg/ml for rBoIFN-IU and 124.9 +/- 40.4 pg/ml for rBoIFN-IM. It is concluded that rBoIFN-alpha can reduce oxytocin-induced PGFM release and may therefore extend the lifespan of the corpus luteum by interfering with events leading to luteolytic release of PGF from the uterus. Administration of rBoIFN-alpha can cause acute changes in body temperature and circulating concentrations of progesterone that become less severe after repeated exposure to rBoIFN-alpha.
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PMID:Alteration of oestrous cycle length, ovarian function and oxytocin-induced release of prostaglandin F-2 alpha by intrauterine and intramuscular administration of recombinant bovine interferon-alpha to cows. 178 57

Bovine conceptuses exert an antiluteolytic (anti-PGF-2 alpha) effect on the uterus by decreasing the secretion of PGF that results in maintenance of the CL. Basal and oxytocin-stimulated secretion rates of PGF from perifused endometrium are lower at Day 17 of pregnancy than at Day 17 of the oestrous cycle, probably because of increased amounts of an intracellular, endometrial PG-synthesis inhibitor which is present in the 100,000 g cytosolic supernatant, is proteinaceous, and acts in a noncompetitive manner with respect to arachidonic acid. The antiluteolytic signal in bovine conceptus secretory proteins (bCSP) is bovine trophoblast protein-1 (bTP-1), a group of 7 isomers of N-linked glycoproteins in two size classes (22,000 high-mannose and 24,000 complex types) that are related immunologically to ovine trophoblast protein-1 (oTP-1). Incubation of endometrial explants of Day-17 cyclic cows with bCSP and bTP-1 induced PG-synthesis inhibitor activity and reduced PGF secretion. Intrauterine infusion of bTP-1 from Days 15.5 to 21 extended the interoestrous interval from 19.5 to 26.0 days. Intrauterine infusion of interferon-alpha I 1 through the cervix from Day 15.5 to 21 extended the interoestrous interval from 22.8 to 26.8 days. Platelet-activating factor (PAF) and interferon-alpha I 1 alter PG secretion of endometrial explants (PAF decreases PGF and increases PGE-2; interferon-alpha I 1 does not alter PGF and increases PGE-2 secretion) but neither induces activity of the endometrial PG-synthesis inhibitor. In conclusion, bovine conceptuses exert a paracrine effect through the secretion of bTP-1 to induce an endometrial intracellular inhibitor of PGF synthesis.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:Antiluteolytic effects of bovine trophoblast protein-1. 281 Feb 37

Three groups of intact hinds (n = 10-18) and one group of ovariectomized hinds were treated with progesterone by mean, of Controlled Internal Drug Releasing (CIDR) devices for 13 days (device removal = Day 0). Group 1 served as controls; group 2 received injections of 4 mg recombinant bovine interferon-alpha,1 twice daily from Days 13 to 21; group 3 was run with a stag from Days 0 to 3, and all hinds were subsequently diagnosed pregnant; group 4 (ovariectomized) was treated with CIDR devices and estradiol to mimic steroid secretion during the estrous cycle. Progesterone profiles were determined from thrice-weekly plasma samples from Days -13 to 28. Rectal temperature was measured in a subset of groups 1 and 2 from Days 9 to 21. Oxytocin-induced prostaglandin F2 alpha release was measured in a subset of groups 1, 2, and 4 on Days 2, 4, 10, 16, and 18. Data are presented as means +/- SEM. Exogenous interferon delayed luteolysis (> or = 28 vs. 21.2 +/- 0.55 days, P < 0.0005) and induced transient pyrexia after the first injection (39.89 +/- 0.11 vs. 38.88 +/- 0.19 degrees C, p < 0.0005). Incidence of oxytocin-induced PGF2 alpha release in control hinds was greater on Days 2 and 18 than on Days 4 and 10 (8/8 and 7/8 vs. 3/8 and 0/8, respectively; p < 0.05) and was greater in control than in interferon-treated hinds on Days 16 and 18 (5/8 and 7/8 vs. 1/8 and 1/8, respectively; p < 0.05). Profiles of plasma progesterone concentration and oxytocin sensitivity in steroid-treated ovariectomized hinds did not differ from those in control hinds. These results suggest that steroid-controlled uterine oxytocin sensitivity is important in luteolysis and is suppressed by the administration of interferon, the putative embryonic pregnancy recognition signal in red deer.
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PMID:Exogenous interferon delays luteal regression in red deer hinds (Cervus elaphus) by suppressing steroid-induced endometrial oxytocin sensitivity. 887 4

Three experiments were carried out to investigate the secretion of luteolytic hormones in red deer hinds during the oestrous cycle, early pregnancy and after administration of interferon, the putative pregnancy recognition signal. Three groups of hinds (n = 8-9 per group) were treated with progesterone-impregnated intravaginal controlled internal drug releasing (CIDR) devices for 13 days (device withdrawal = day 0). Group 1 (n = 9) served as controls; Group 2 (n = 8) received injections of 4 mg recombinant bovine interferon-alpha 1 l twice a day on days 13-18; Group 3 (n = 9) were run with a fertile stag on days 0-3. Plasma samples collected each day on days 16-23 were analysed for progesterone. Plasma samples collected each hour for 16 h on days 4, 10, 16, 18 were analysed for oxytocin and the prostaglandin F2 alpha (PGF2 alpha) metabolite (PGFM). Plasma progesterone concentrations declined to < 1 ng ml-1 between days 18 and 25 in control hinds indicating that luteolysis had occurred, whereas there was no endocrine evidence of luteolysis in interferon-treated or pregnant hinds. Control hinds (6/9) exhibited synchronous pulses of oxytocin and PGF2 alpha secretion on day 18, a greater proportion than on any other day in these hinds or on any day in the interferon-treated or mated hinds (P < 0.05). In a second experiment, close synchrony in secretion of oxytocin and PGF2 alpha pulses was evident in an unmated hind when samples were collected every 12 min on day 18. In a third experiment, oxytocin-induced PGF2 alpha secretion was potentiated by oxytocin administration at an interval of 1 h and inhibited by administration at a 6 h interval (P < 0.05). These results suggest that synchronous pulsatile secretion of oxytocin and PGF2 alpha induces luteolysis and is suppressed by pregnancy or administration of interferon. Oxytocin-induced alterations in uterine oxytocin sensitivity may underlie the pulsatile nature of luteolytic hormone secretion in red deer.
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PMID:Effect of pregnancy and exogenous interferon on synchronous pulsatile release of oxytocin and luteolytic prostaglandin F2 alpha in red deer (Cervus elaphus). 946 99