Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P01034 (
cystatin C
)
3,397
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Recently, we showed that mechanical stress on scleractinian (stony) corals caused a rapid release of antibacterial material (referred to as coral antibacterial activity, or
CAA
), which killed various bacterial species, including the coral pathogen Vibrio coralliilyticus. We now report on studies on the regulation of
CAA
release from stressed scleractinian corals. Corals can repeatedly release highly active
CAA
as a result of sequential stress inductions.
Coral
fragments were transferred 19 times from one beaker into another with a stress induction each time after 10 min. There was a decrease in the level of antibacterial activity released during the first four to five transfers. After the fifth transfer, the corals kept releasing
CAA
for the rest of the experiment with no significant decrease. Apparently, the release of
CAA
is downregulated by feedback inhibition, depending on the concentration of
CAA
in the surrounding water. By separating
CAA
-treated V. coralliilyticus from the surrounding water, it was shown that
CAA
was bound irreversibly to bacterial cells in a stoichiometric manner. Approximately 4 x 10(2) bacterial cells were sufficient to bind 1 U of
CAA
. Resident coral bacteria were more resistant to
CAA
than bacteria isolated from seawater, suggesting an ecological role for
CAA
.
CAA
release was obtained from corals after removal of the mucus layer, and the mucus itself contained antibacterial activity.
...
PMID:Regulation of release of antibacterials from stressed scleractinian corals. 1947 57
