Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P01034 (
cystatin C
)
3,397
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Cystatins are reversible cysteine protease inhibitor proteins. They are known to play important roles in controlling cathepsins, neurodegenerative disease, and in immune system regulation. Production of recombinant cystatin proteins is important for biochemical and function characterization. In this study, we cloned and expressed human stefin A, stefin B and
cystatin C
in Escherichia coli. Human stefin A, stefin B and
cystatin C
were purified from soluble fraction. For
cystatin C
, we used various chaperone plasmids to make
cystatin C
soluble, as it is reported to localize in inclusion bodies.
Trigger
factor, GroES-GroEL, DnaK-DnaJ-GrpE chaperones lead to the presence of
cystatin C
in the soluble fraction. Immobilized metal affinity chromatography, glutathione sepharose and anion exchange chromatography techniques were employed for efficient purification of these proteins. Their biological activities were tested by inhibition assays against cathepsin L and H3 protease.
...
PMID:Efficient expression and purification of biologically active human cystatin proteins. 2648 Dec 72
