Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P01034 (
cystatin C
)
3,397
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Murine monoclonal antibodies against the major cysteine proteinase inhibitors of human biological fluids,
cystatin C
and kininogen, were produced. The
cystatin C
antibody, HCC3, with a Ka of 2 x 10(7) l/mol, increased the inhibition of papain by
cystatin C
and was suitable for use in immunoblotting, immunohistochemistry and in the construction of a sensitive sandwich enzyme immunoassay for quantification of
cystatin C
. It recognized not only free
cystatin C
but also
cystatin C
in complexes with cysteine proteinases. The kininogen antibody,
HK4
, was directed against the third, cysteine proteinase inhibitory domain of the heavy chain of kininogen (Ka = 1 X 10(7) l/mol), but did not influence the papain inhibitory activity of kininogen. It reacted with free kininogen as well as kininogen in complex with cysteine proteinases. Both antibodies could be used for the production of specific immunosorbents.
...
PMID:Production, characterization and use of monoclonal antibodies against the major extracellular human cysteine proteinase inhibitors cystatin C and kininogen. 306 78
