Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P00790 (
PGA
)
2,475
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The effects of various proteolytic enzymes on the high molecular weight protein (
connectin
) present in a direct sodium dodecyl sulfate extract of myofibrils from chicken breast muscle were studied in detail. To keep the high molecular weight proteins intact, myofibrils had to be prepared in the presence of EGTA. Trypsin, chymotrypsin, papain, and nagarse readily hydrolyzed
connectin
(doublet band of titin) and the band 3 protein (N2-line protein).
Pepsin
did not attack
connectin
, but digested the band 3 protein and myosin. Calcium-activated neutral proteinase hydrolyzed the band 3 protein, leaving
connectin
intact. On the other hand, serine protease digested
connectin
but not the band 3 protein.
...
PMID:Connectin, an elastic protein of muscle. Effects of proteolytic enzymes in situ. 702 43
