Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P00790 (PGA)
 2,475 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The rates of proteolytic breakdown for native human hemoglobin (Hb) in CNmet-and oxy-forms, for isolated native alpha- and beta-chains of human Hb with deprotected SH-groups and for apo-Hb--globin at constant temperature 20 degrees as well as for metHb and globin in the temperature range 4-25 degrees were studied. The proteolysis of oxy-forms of proteins was performed in the presence of CN- to prevent the appearance in solution of quickly splitted aqua and hydroxy met-forms. Pepsin (at pH 5.5), trypsin (at pH 7.0 and 8.5) and protease VI (pronase) (at pH 7.0 and 8.5) were used as proteases. The rate of proteolysis was registered simultaneously by proteolysate precipitation in concentrated salt solutions (to determine the content of the native form), by precipitation in aqueous solution of trichloroacetic or perchloric acid and by colouring the terminal NH2-groups by ninhidrin in the total proteolysate. For most cases the data of all the three independent methods fell on a single kinetic curve, each pair protein--protease being represented by their individual curves. Therefore the breakdown of all the protein studied possesses a burst-like ("one-by-one", "all-or-none") character. The protein resistance to the attack by proteolytic enzymes increases in the following order: globin less than oxy-alpha-chain less than metHb less than oxy-beta-chain less than HbO2 congruent to CNmetHb. The use of control repeated proteolysis has made it possible to prove that differences in the rate of proteolytic degradation are not the consequence of spontaneous denaturation of the least unstable forms of proteins in the course of proteolytic reaction but are predetermined by the conformational state of the native macromolecule.
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PMID:[Proteolytic degradation of native hemoglobin and its constituent parts--isolated subunits and globin. I. Kinetic data and the character of the process of the breakdown of native forms]. 681 53

Selective extraction of specific cell components by enzyme or acid hydrolysis is possible from ultrathin sections for electron microscopy and parallel 2 micro sections for light microscopy of tissues fixed in formalin and embedded in a water-soluble polyepoxide, product X133/2097. Normal rat tissues fixed 15 minutes in formalin at 3 degrees C are more rapidly digested by proteinases than those fixed for the same length of time at 20 degrees C. Trypsin selectively attacks the nuclear chromatin and the ribonucleoprotein particles of the ergastroplasm, whereas mitochondria and zymogen granules resist tryptic digestion. Pepsin rapidly attacks the mitochondria and zymogen granules. The ergastoplasm and nucleus at first resist peptic digestion, but in time the entire cytoplasm and interchromatinic portion of the nucleus are attacked. Ribonuclease abolishes cytoplasmic basophilia in 2 micro sections, but parallel ultra-thin sections, stained with uranyl acetate and examined in the electron microscope, show no change in the ribonucleoprotein particles of the ergastoplasm. Desoxyribonuclease alone had no effect, but after pretreatment of the sections with pepsin or hydrochloric acid, desoxyribonuclease specifically attacked the nuclear chromatin. Nucleic acid-containing structures in the sections are gradually disintegrated by perchloric acid or hydrochloric acid.
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PMID:Ultrastructural cytochemistry. Enzyme and acid hydrolysis of nucleic acids and protein. 1376 Feb 8