Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P00790 (
PGA
)
2,475
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The ability of cathepsin D, chymosin, pepsin and renin to produce
endothelin-1
(
ET-1
) from proendothelin-1 (proET-1) was compared. No significant conversion was observed when proET-1 was incubated with up to 1 U of renin for 15 min at 37 degrees C. Cathepsin D generated, as well as degraded,
ET-1
rapidly. Net production of
ET-1
reached a maximum when 0.003 U of cathepsin D was used, and about 16% of the initial proET-1 was detected as
ET-1
by HPLC.
Pepsin
up to 1 U converted proET-1 into
ET-1
dose-dependently with a maximum of 71% conversion. A further increase of the amount of pepsin in the reaction mixture produced nonspecific cleavage of
ET-1
. Less than 10% of
ET-1
remained in the presence of 15 U of pepsin. Chymosin also generated
ET-1
dose-dependently, and a complete conversion was obtained at 1 U of enzyme. Greater than 1 U of chymosin only slightly degraded
ET-1
; at least 80% of
ET-1
was still present when 15 U of chymosin was included in the assay. Other properties associated with the conversion of proET-1 into
ET-1
by chymosin were investigated. Similar to authentic
ET-1
, the product of chymosin treatment caused contraction of isolated rabbit aortic rings, and pre-incubation of chymosin with pepstatin A abolished this contractile response.(ABSTRACT TRUNCATED AT 250 WORDS)
...
PMID:Conversion of proendothelin-1 into endothelin-1 by aspartylproteases. 822 77
