Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P00790 (PGA)
 2,475 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A highly sensitive radioimmunoassay for the measurement of plasma prostaglandins A and B, expressed in equivalents of PGA1, is described. This method was used for the measurement of prostaglandins A and B (PGA/B) in 23 healthy volunteers and 25 hypertensive patients. The PGA/B concentration in peripheral venous plasma of 23 healthy normotensive subjects is 115 +/- 15 pg/ml. The repeated measurement of the same plasma samples kept frozen for 60 days at -20 degrees C shows mean 194% increase of PGA/B concentration. The major site of synthesis of PGA/B seems to be the kidney. However in two patients PGA/B concentration in arterial blood was greater than in venous blood suggesting the possibility of cardio-pulmonary synthesis. The major site of inactivation is the hepatic circulation, as PGA/B concentration in hepatal venous blood is by 30% lower than in vena caval blood. The arterial concentration is 3% lower than venous PGA/B demonstrating very low pulmonary inactivation. Therefore the prostaglandins of the A and B series may represent a "circulating hormone". The plasmatic PGA/B is significantly increased in reno-vascular and essential hypertension.
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PMID:Radioimmunoassay of prostaglandins A and B in human blood. 96 52

The relative stability of Prostaglandins (PGs) E1, E2 and F1alpha in cultures of BALB/c 3T3 and SV3T3 cells has been evaluated using 3 different approaches. First, total recovery of tritium in the ethyl acetate phase following incubation and extraction of PGF1alpha and PGE1 demonstrated greater stability for PGF1alpha (88.8%) than PGE1 (65.9%). Second, analysis of incubated, extracted, tritiated PGs by thin layer chromatography revealed decreases of up to 23% in the PGE zone following incubation of 3H-PGE1. With increasing time of incubation, decreases in the PGE zone were accompanied by increase in PGA-like compounds. 3H-PGF1alpha demonstrated greater stability, having greater than 90% recovery of the tritium in the PGF zone. A third approach to the assessment of PG stability in culture was the comparison of the production of individual PGs by radioimmunoassay (RIA). The data obtained by RIA indicated a lag in the increase of PGA and PGB, until an initial rise in PGE was noted, suggesting that PGA and PGB may be secondary products arising from PGE which exhibits only partial stability in culture. By employing two RIAs, one for total PGE and one for PGA and PGB, the composite determination PG [E + (A + B)] can be used to provide a more meaningful determination of PG production because of the instability of the PGs. On the other hand, individual determinations are helpful in assessing the stability of PGEs in cell cultures.
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PMID:The simultaneous use of two prostaglandin radioimmunoassays employing two antisera of differing specificity. II. Relative stability of prostaglandins E1, E2 and F1alpha in cell cultures of BALB/c 3T3 and SV3T3 mouse fibroblasts. 98 8

Postpartum sexual abstinence time can be safely shortened for most patients when episiotomy repair is done meticulously with fine PGA suture on small needles. The time preferred by patients for resumption of intercourse seems to be between the second and third postpartum week. We have seen no ill effects from this, and we feel that sexual intercourse at these early dates does not influence the healing of the episiotomy in any way.
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PMID:Decreasing postpartum sexual abstinence time. 98 1

A total of 206 patients were subjected to operation for acute appendicitis through a pararectal laparotomy. Mersilene was used in 101 and polyglycolic acid (PGA, Dexon) in 105 patients as buried sutures for the closure of the abdominal wall, including the fascial layer. Wound complications developed after Mersilene in 23 and after PGA in 10 cases. PGA did not lead to an increased frequency of early wound infections. Granulomata or fistulae developed after Mersilene in 18 patients, but not in any of the cases in which PGA was used. Wound rupture and incisional hernia did not occur in any of the patients in the two groups.
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PMID:A comparison of Mersilene and polyglycolic acid sutures in pararectal abdominal wounds after appendectomy. 103 60

1. A number of folates labelles with 14-C were administered orally to rats, at various doses, and urinary, faecal and hepatic folates examined. 2. 10-Formylpteroylmonoglutamic acid ( 10CHO--PGA) entered the folate pool very slowly, and is thought to be relatively ineffective in nutrition. 3. 10-Formyl[2-14-C]tetrahydrofolic acid (10CHO--[2-14-C]THF) entered the folate pool very rapidly. 5-Methyl[2-14-C]tetrahydrofolate (5CH3--[2-14-C]THF) was the major urinary folate. 4. 5-Formyl[2-14-C]tetrahydrofolic acid (5CHO--[2-14-C]THF) entered the folate pool only to a small extent. 5CHO--[2-14-C]THF, given intravenously, produced no urinary 5CH3--[2-14-C]THF in the first 6 h. 5. 5,10-Methylidyne[2-14-C]tetrahydrofolic acid was metabolized to an extent which was dependant on the dose. At doses of 3 and 30 mug/kg body-weight, 5CH3--[2-14-C]THF represented 5-4 and 20% respectively of urinary folates anf for 10CHO--[2-14]PGA, the values were 16% of total urinary folates after the higher dose, and 78-5% after the lower dose. 6. Results obtained for the metabolism of 5CH3--THF varied depending on the position of the labelling: 5-14-CH3-THF gave no labelled urinary folate, the methyl group being lost rapidly. When 5CH3--[2-14-C]THF was given, it appeared as the major urinary folate. 7. Folates found in the liver after oral administration of labelled folates were identified by thin-layer chromatography; only folate monoglutamates were identified.
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PMID:The metabolism of some folates in the rat. 107 38

In experiments in which blood was cross-circulating in rats, the blood pressure of the recipient dropped while that of the donor rose, following the increase of the circulating blood volume, produced by infusion either of saline or blood. The phenomenon was almost imperceptible when binephrectomized animals were used. In experiments in which the blood-bathed organ technique was used, prostaglandin-like substances were detected, released during the rise of the blood pressure, produced by the same stimulus (the expansion), in anaesthetized rats. A significant difference was found between the prostaglandin-like substances detected using the blood-bathed organ technique, in normal rats (5.387 ng per ml of blood plus or minus 0.288 = SEM) and those detected in binephrectomized rats (3.202 ng per ml of blood plus or minus 0.330, p smaller than 0.025). The biologically active substances detected in 25 ml of blood collected during expansion, while the assay organs showed a prostaglandin-like activity, were found to have the chromatographic behaviour and the bioassay properties of PGA, PGE and PGF series. A great quantity of the biologically active substances, having the chromatographic behaviour and the bioassay properties of PGA, PGS and PGF was detected in the rat renal medulla. Sufficient quantities of the released prostaglandin-like substances could escape the pulmonary vascular bed in this species of animal. It was concluded that a great quantity of the released prostaglandin-like substances came from the kidney and their release by this particular mechanism suggested that they play an important homeostatic role on the blood pressure, blood volume, and sodium and water balance regulation.
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PMID:[Origin, nature, role and fate of prostaglandins liberated during the expansion of intravascular space in the anesthetized rat]. 109 32

Monofilaments of the absorbable synthetic thread Dexon made from PGA have properties similar to nylon 10-0. Experiments with intra-lamellar implantation of the monofilament in rabbit corneas showed the same good material tolerance as nylon 10-0. Perforating corneal wounds were closed with interrupted and running sutures from PGA monofilaments, respectively. One week post-operatively, increased intraocular pressure caused a wound rupture but not a thread rupture. Two weeks post-operatively an increased intraocular pressure of more than 300 mm Hg could no longer rupture the wound. Slit lamp and histological examinations revealed symptoms of absorption after the second week. According to our findings, PGA monofilaments may be suitable for corneal surgery in patients.
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PMID:Animal experiments on an absorbable synthetic thread made of polyglycolic acid (PGA) in corneal surgery. 109 29

A highly concise review is presented of the experimental and clinical literature concerning polyglycolic acid suture material, marketed for clinical use for the first time in 1970. It is a synthetic, completely absorbable, non-toxic product, which not only offers a number of advantages over catgut, but may also be used where non-absorbable materials were formerly applied exclusively. Our favorable experiences in 500 patients, who were subjected to many different kinds of general surgical operations, are in agreement with those published in the literature. We have gained the impression that suture fistulization, which may lead to protracted morbidity after use of non-absorbable material, has decreased in incidence and severity after the use of PGA suture material. This is a definite advantage.
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PMID:Experience with the use of polyglycolic acid suture material (Dexon) in 500 surgical patients. 109 79

The renal prostaglandins PGS2 and PGE2 possess potent antihypertensive and vasodepressor activity. The mechanism of blood pressure lowering effect is through peripheral arteriolar dilation with a fall in total peripheral resistance. PGA unlike PGE escape degradation by the lung and thus could circulate as antihypertensive hormones. Since plasma PGA levels rise in humans on a low sodium intake, it has been postulated that the beneficial effects of a low sodium diet in some hypertensives may be the result of an increase in peripheral vasodilating PGA. Support that plasma PGA may be a regulator of systemic blood pressure is also derived from the fact a PGA-secreting renal tumor was associated with a fall in blood pressure and a rise in plasma PGA in a previously hypertensive woman. The removal of the tumor resulted in a return of blood pressure to elevated levels and a concomitant fall in PGA. Recently, a number of human patients with essential hypertension have been infused with PGA1 and PGA2. It was observed that there was an initial increase in renal blood flow, sodium and water excretion which was associated with no change in the elevated blood pressure. When blood pressure ultimately fell, there was a return of renal blood flow, sodium and water excretion to preinfusion levels. It would appear that PGA compounds act as 'ideal' antihypertensive agents since they favorably effect renal resistance, sodium and water homeostasis, plasma volume, total peripheral resistance, blood pressure and indirectly cardiac output through baroreceptor stimulation, all factors known to be important in etiology in human hypertension.
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PMID:Renal prostaglandins. 110 Oct 92

Polyglycolic acid sutures were used in 239 cases of intraocular surgery (164,6-0 size, 75, 7-0 size). Most of these (226) were cataract extractions. The results were excellent, with minimal complications. The 6-0 suture was somewhat stiff and associated with some tissue drag. The 7-0 size overcame these deficiencies and was considered the closest approach to date to the ideal absorbable suture. In addition, these appears to be an inhibition to bacterial penetration by PGA.
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PMID:Further use of polyglycolic acid (Dexon) sutures in cataract and other intraocular surgery. 111 10


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