Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P00790 (PGA)
 2,475 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The influence of tissue pretreatment on the PAP immunostaining for type I and III collagens and tenascin was studied in formalin-fixed and paraffin-embedded human tooth germs at the 24th and 25th weeks of fetal life. Three variables were considered: the type of buffer used (PBS or Tris), pepsin digestion and the use of normal serum as a blocking agent prior to immunostaining. All three proteins needed an enzymatic digestion to be intensely revealed. Pepsin promoted, even at low concentrations, an intracellular staining of type I collagen in the secretory odontoblasts and in the pulpal fibroblasts. Normal serum partially blocked unspecific immunoreaction when polyclonal rabbit antibodies were used. The Tris buffer increased the staining intensity of the three macromolecules and revealed an unusual tenascin-like immunoreactivity in the ameloblasts. This study demonstrated that pepsin digestion and the use of normal serum and different buffers may influence the immunoreactivity of ECM proteins.
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PMID:The influence of tissue pretreatment on the immunohistochemical demonstration of type I and III collagens and tenascin in fetal human tooth germs. 769 Nov 42

The effects of fixatives and pretreatment on the immunofluorescence of whole mount specimens prepared for confocal laser scanning microscopy (CLSM) were examined. Intact villi were obtained from the proximal small intestine of mice fixed with 4% paraformaldehyde (4P) or 0.5% paraformaldehyde and 15% of saturated picric acid (PPa). Before immunostaining for laminin and tenascin, each specimen was pretreated with deoxycholate, while some 4P-fixed specimens received further pepsin pretreatment. Regardless of the fixatives employed, laminin and tenascin showed adequate immunofluorescence. Without pepsin pretreatment, the 4P-fixed specimens emitted conspicuous background fluorescence, and immunofluorescence was weak in the lamina propria. Pepsin pretreatment reduced the background fluorescence, but also diminished the immunofluorescence, especially that of tenascin. The PPa-fixed specimens displayed intense immunofluorescence of laminin and tenascin with very little background, even deeply within the lamina propria. When the PPa-fixed specimens were immunostained for vasoactive intestinal peptide, immunopositive nerve fibres were observed within the lamina propria. Ultrastructural investigation of the PPa-fixed specimens revealed that membranous structures in all cells were almost lost while tissue architecture was well preserved. These results indicate that PPa fixation and pretreatment with deoxycholate are suitable for preparing whole mount specimens for CLSM studies.
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PMID:A note on the preparation of whole mount samples suitable for observation with the confocal laser scanning microscope. 915 Aug 2