Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P00750 (
PLA
)
16,800
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Mineralization is a key process in the formation of bone and cartilage in vertebrates, involving the deposition of calcium- and phosphate-containing hydroxyapatite (HA) mineral within a collagenous matrix. Inorganic phosphate (P
i
) accumulation within matrix vesicles (MVs) is a fundamental stage in the precipitation of HA, with
PHOSPHO1
being identified as the principal enzyme acting to produce P
i
PHOSPHO1
is a dual-specific phosphocholine/phosphoethanolamine phosphatase enriched in mineralizing cells and within MVs. However, the source and mechanism by which
PHOSPHO1
substrates are formed before mineralization have not been determined. Here, we propose that 2 enzymes-phospholipase A2 (
PLA
2
) and ectonucleotide pyrophophatase/phosphodiesterase 6 (ENPP6)-act in sequence upon phosphatidylcholine found in MV membranes to produce phosphocholine, which
PHOSPHO1
can hydrolyze to liberate P
i
This hypothesis is supported by evidence that both enzymes are expressed in mineralizing cells and data showing that phosphatidylcholine is broken down in MVs during mineralization. Therefore,
PLA
2
and ENPP6 activities may represent a key step in the mineralization process. Further functional studies are urgently required to examine their specific roles in the initiation of skeletal mineralization.-Stewart, A. J., Leong, D. T. K., Farquharson, C.
PLA
2
and ENPP6 may act in concert to generate phosphocholine from the matrix vesicle membrane during skeletal mineralization.
...
PMID:PLA
2
and ENPP6 may act in concert to generate phosphocholine from the matrix vesicle membrane during skeletal mineralization. 2886 58
