Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P00750 (PLA)
 16,800 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The crucial role of non-plasminogen dependent serine proteinases is tissue invasive and cytolytic functions of Walker 256 cancer cells has been documented using a rat urinary bladder invasion and a 125I-labelled fibroblast cytolysis assay. The invasive capacity of these cancer cells was abrogated by non toxic concentrations of the serine proteinase inhibitors, diisopropylfluorophosphate and phenylmethylsulfonylfluoride, but not by metallo or cysteine proteinase inhibitors. Although tumour cell collagenase activity and plasminogen activator were demonstrated, these proteolytic enzymes were not essential in these in vitro assays. These results suggest that different categories of proteinases play specific roles in the complicated process of cancer invasion.
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PMID:Role for different cell proteinases in cancer invasion and cytolysis. 299 66

JCV induces glioblastomas in owl monkeys 18-24 months or longer following intracranial inoculation (W. London, S. Houff, D. Madden, D. Fuccillo, M. Gravell, W. Wallen, A. Palmer, J. Sever, B. Padgett, D. Walker, G. Zu Rhein, and T. Ohashi, 1978, Science 201, 1246-1248). Cells from one brain tumor, owl monkey 26, were successfully established in culture and analyzed for phenotypic characteristics generally associated with persistence and expression of the papovavirus early region of its genome. Owl monkey 26 cells demonstrated nuclear JCV T protein detected by either SV40 hamster tumor sera or PAb 108, a monoclonal antibody to SV40 T protein. However, the JCV T protein was not detected in a complex with the host cell p53 protein as judged by immunoprecipitation using PAb 122, a monoclonal antibody directed to the mammalian p53 cellular protein. These cells also demonstrated increased plasminogen activator secretion and actin cable disorganization properties not before reported for JCV-induced tumor or transformed cells. Of the primate papovaviruses, JCV is unique in its ability to induce brain tumors in these primates. JCV early gene expression can be shown to persist in brain tumor cells once established in culture and correlates with cell phenotypes typical of papovavirus malignant transformation even though the time between virus inoculation and tumor development is usually several years.
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PMID:JC virus-induced owl monkey glioblastoma cells in culture: biological properties associated with the viral early gene product. 608 49

Three kinds ( EFF-1, EFF-2 and EFF-3) of fibrinolytic factor were separated by ammonium sulphate precipitation, DEAE-cellulose and preparative PAGE electrophoresis from female Eupolyphaga sinensis Walker. Their molecular weights were proved to be 41kd, 32.9 kd and 30.6 kd respectively with SDS-PAGE electophoresis. Their Activities as plasminogen activator were 171.3 U/mg, 234.0 U/mg and 148.5 U/mg. In addition, EFF-2 and EFF-3 were not only fibrinolytic activities but also have plasminogen activator on fibrinous plate lacked of plasminogen . There had been no such components of plasminogen activator and fiberinolytic enzyme from Eupolyphaga sinensis reported yet.
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PMID:[Purification and activity calculated of fibrinolyric factors from Eupolyphaga sinensis]. 1707 34