Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P00750 (PLA)
 16,800 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

In the present work, we propose a new multifunctional micelle, co-self-assembled from different monofunctional copolymers, for tumor targeting and fluorescent and electron spin resonance (ESR) dual detection. Firstly, a poly(D,L-lactic acid)-b-poly(N-isopropyl methacrylamide-co-N-isopropylmaelic acid-co-10-undecenoic acid)-b-poly(N-acryloxysuccinimide) (PLA-PNNUA-PNAS) copolymer, with pH-dependent thermo-responsive properties, was synthesized. The copolymer was synthesized using reversible addition fragmentation chain transfer (RAFT) polymerization method, after which it was further used as a parent copolymer to combine with folic acid (FA), fluorescein isothiocyanate (FITC) and 4-amino-2,2,6,6-tetramethylpiperidin-1-oxyl (4-NH2-TEMPO), respectively, resulting into three new monofunctional copolymers. Finally, the multifunctional copolymer micelle was easily then fabricated, through co-self-assembly, using the monofunctional copolymers. The results from in vitro cell assays indicated that the proposed micelle was able to provide desired multifunctional properties, including tumor specific targeting and fluorescent and ESR dual detection. Additionally, the parent copolymer allowed conjugation with other ligands to prepare them for more functional copolymers attachment for future potential applications. More importantly, the multifunctional properties of the copolymer micelles could be rationally tailored, able for given purposes.
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PMID:Three monofunctional copolymers into one multifunctional Micelle: Part 1. Preparation and properties. 2473 32

In this paper, the doxorubicin (DOX)-loaded micelles were prepared based on a novel folic acid conjugated pH-dependent thermo-sensitive copolymer poly(D,L-lactic acid)-b-poly(N-isopropyl methacrylamide-co-N-isopropylmaelic acid-co-10-undecenoic acid) (PLA-PNNUA-FA) constructed to provide an active targeting drug delivery and triggered drug release system. The micelles were able to target tumors through the interaction between folic acid and its receptors which are overexpressed on the tumor cell membrane, and achieved pH-dependent thermo induced drug release in the intracellular mild acidic media such as endosomes and lysosomes after the micelles enter the cells. The results of cell assays and animal experiments showed that the micelles exhibited obvious tumor penetration efficiency in vivo, also improved DOX cell uptake and cytotoxicity in vitro. It was suggested that copolymer PLA-PNNUA-FA might be a potential targeted drug carrier to deliver chemotherapeutic drugs achieving better efficacy of chemotherapy.
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PMID:Doxorubicin-Loaded Micelles Based on Folic Acid Conjugated pH-Dependent Thermo-Sensitive Copolymer: In Vitro and In Vivo Evaluation. 2636 16