Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts: Gene/Protein Disease Symptom Drug Enzyme Compound   Target Concepts: Gene/Protein Disease Symptom Drug Enzyme Compound

---

Query: UNIPROT:P00750 (PLA)
16,800 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Bee venom phospholipase A₂ (PLA₂) has potential for significant morbidity. Ruthenium (Ru)-based carbon monoxide releasing molecules (CORM) inhibit snake venoms that are anticoagulant and contain PLA₂. In addition to modulating heme-bearing proteins with carbon monoxide, these CORM generate reactive Ru species that form adducts with histamine residues resulting in changes in protein function. This study sought to identify anticoagulant properties of bee venom PLA₂ via catalysis of plasma phospholipids required for thrombin generation. Another goal was to determine if Ru-based CORM inhibit bee venom PLA₂ via carbon monoxide release or via potential binding of reactive Ru species to a key histidine residue in the catalytic site of the enzyme. Anticoagulant activity of bee venom PLA₂ was assessed via thrombelastography with normal plasma. Bee venom PLA₂ was then exposed to different CORM and a metheme forming agent and anticoagulant activity was reassessed. Using Ru, boron and manganese-based CORM and a metheme forming agent, it was demonstrated that it was unlikely that carbon monoxide interaction with a heme group attached to PLA₂ was responsible for inhibition of anticoagulant activity by Ru-based CORM. Exposure of PLA₂ to a Ru-based CORM in the presence of histidine-rich human albumin resulted in loss of inhibition of PLA₂. Ru-based CORM likely inhibit bee venom PLA₂ anticoagulant activity via formation of reactive Ru species that bind to histidine residues of the enzyme.
...
PMID:The anticoagulant effect of Apis mellifera phospholipase A₂ is inhibited by CORM-2 via a carbon monoxide-independent mechanism. 3167 16

Presented herein is the case of a 37-year-old male who was scheduled for an anterior decompressive laminectomy after suffering trauma to the cervical area (C6-C7). An intraoperative acute pulmonary embolism (APE) was suspected after persistent hypoxemia and a decreased end-tidal CO2 that was refractory to proper management. After 6 intraoperative episodes of cardiac arrest that followed, intravenous alteplase (thrombolytic therapy) was administered, and the patient was stabilized without major complications. Eventually, APE was successfully diagnosed and treated. The experience served as reference for the diagnosis and management of APE under general anesthesia.
...
PMID:Complete Cognitive Recovery and Survival From Massive Pulmonary Embolism During General Anesthesia after Administration of Alteplase: A Case Report. 3238 71

Bionanocomposites based on poly (lactic acid) (PLA) and silica aerogel (SiA) were developed by means of melt extrusion process. PLA-SiA composite films were plasticized with 15 wt.% of acetyl (tributyl citrate) (ATBC) to facilitate the PLA processability as well as to attain flexible polymeric formulations for films for food packaging purposes. Meanwhile, SiA was added in four different proportions (0.5, 1, 3 and 5 wt.%) to evaluate the ability of SiA to improve the thermal, mechanical, and barrier performance of the bionanocomposites. The mechanical performance, thermal stability as well as the barrier properties against different gases (carbon dioxide, nitrogen, and oxygen) of the bionanocomposites were evaluated. It was observed that the addition of 3 wt.% of SiA to the plasticized PLA-ATBC matrix showed simultaneously an improvement on the thermal stability as well as the mechanical and barrier performance of films. Finally, PLA-SiA film formulations were disintegrated in compost at the lab-scale level. The combination of ATBC and SiA sped up the disintegration of PLA matrix. Thus, the bionanocomposites produced here show great potential as sustainable polymeric formulations with interest in the food packaging sector.
...
PMID:Hybrid Biocomposites Based on Poly(Lactic Acid) and Silica Aerogel for Food Packaging Applications. 3314 3

Exposing polymers to high-pressure and supercritical CO2 is a useful approach in polymer processing. Consequently, the mechanisms of polymer-polymer interaction under such conditions are worthy of further investigation. Two-dimensional (2D) correlation analysis and 2D disrelation mapping were applied to datasets of PCL(Polycaprolactone)/PLA (Poly(lactic acid)) blend with or without high-pressure CO2 obtained by in situ ATR-FTIR spectroscopic imaging. The relatively weak dipole-dipole intermolecular interactions between polymer molecules were visualized through the disrelation maps for the first time. Because of the specially designed polymer interface, the interactions between the same type of polymer molecules and different types of polymer molecules were differentiated. Under exposure to high-pressure CO2, all three types of interactions: interaction between PCL molecules and PLA molecules, interaction between PCL molecules and interaction between PLA molecules become weaker than those in the polymer interface without high-pressure CO2. The resulting increase in the Flory interaction parameter is the main cause of phase separation in the PCL/PLA blend under high-pressure CO2. The findings from this study will be of benefit for polymer processing with high-pressure and supercritical CO2.
...
PMID:EXPRESS: Intermolecular Interactions in the Polymer Blends Under High-Pressure CO₂ Studied Using Two-Dimensional Correlation Analysis and Two-Dimensional Disrelation Mapping. 3323 78

Supercritical carbon dioxide (SC-CO₂) can serve as solvent, anti-solvent and solute, among others, in the field of drug delivery applications, e.g., for the formulation of polymeric nanocarriers in combination with different drug molecules. With its tunable properties above critical pressure and temperature, SC-CO₂ offers control of the particle size, the particle morphology, and their drug loading. Moreover, the SC-CO₂-based techniques overcome the limitations of conventional formulation techniques e.g., post purification steps. One of the widely used polymers for drug delivery systems with excellent mechanical (T_g, crystallinity) and chemical properties (controlled drug release, biodegradability) is poly (lactic acid) (PLA), which is used either as a homopolymer or as a copolymer, such as poly(lactic-co-glycolic) acid (PLGA). Over the last 30 years, extensive research has been conducted to exploit SC-CO₂-based processes for the formulation of PLA carriers. This review provides an overview of these research studies, including a brief description of the SC-CO₂ processes that are widely exploited for the production of PLA and PLGA-based drug-loaded particles. Finally, recent work shows progress in the development of SC-CO₂ techniques for particulate drug delivery systems is discussed in detail. Additionally, future perspectives and limitations of SC-CO₂-based techniques in industrial applications are examined.
...
PMID:PLA/PLGA-Based Drug Delivery Systems Produced with Supercritical CO₂-A Green Future for Particle Formulation? 3323 37


<< Previous 1 2 3 4 5 6 7 8

---