Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:P00750 (
PLA
)
16,800
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
This work reports the purification and biochemical and functional characterization of
ACP
-TX-I and
ACP
-TX-II, two phospholipases A
2
(
PLA
2
) from
Agkistrodon contortrix pictigaster
venom. Both
PLA
2
s were highly purified by a single chromatographic step on a C
18
reverse phase HPLC column. Various peptide sequences from these two toxins showed similarity to those of other
PLA
2
toxins from viperid snake venoms.
ACP
-TX-I belongs to the catalytically inactive K49
PLA
2
class, while
ACP
-TX-II is a D49
PLA
2
, and is enzymatically active.
ACP
-TX-I
PLA
2
is monomeric, which results in markedly diminished myotoxic and inflammatory activities when compared with dimeric K49
PLA
2
s, confirming the hypothesis that dimeric structure contributes heavily to the profound myotoxicity of the most active viperid K49
PLA
2
s.
ACP
-TX-II exhibits the main pharmacological actions reported for this protein family, including in vivo local myotoxicity, edema-forming activity, and in vitro cytotoxicity.
ACP
-TX-I
PLA
2
is cytotoxic to A549 lung carcinoma cells, indicating that cytotoxicity to these tumor cells does not require enzymatic activity.
...
PMID:ACP-TX-I and ACP-TX-II, Two Novel Phospholipases A
2
Isolated from Trans-Pecos Copperhead
Agkistrodon contortrix pictigaster
Venom: Biochemical and Functional Characterization. 3173 3
