Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UNIPROT:P00750 (PLA)
 16,800 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Cell proliferation has been found to correlate with increased secretion of proteinases, such as plasminogen activator, in several different cell populations. In addition, the shape of the cell may also play a role in regulating proteinase secretion. However, the relationship between cell proliferation, cell shape and proteinase secretion has not been studied in diploid epithelial (E) cells cultured from porcine periodontal ligament (PL). We have modified PLE cell shape by physical means, such as growth on less-adhesive substrata and mechanical stretching, and by exposure to cholera toxin and 12-O-tetradecanoylphorbol-13-acetate (TPA). Neutral proteinase and plasminogen activator secretion were found to correlate with cell shape, the more round the cells, the greater the amount of proteinase secreted. PLE cells, stimulated to proliferate by cholera toxin or dibutyryl cyclic AMP, were more spread than control cells, but secreted less neutral proteinase and plasminogen activator. TPA stimulated cell proliferation slightly but, in contrast to cholera toxin, increased cell rounding and the secretion of neutral proteinase and plasminogen activator. Thus proteinase secretion was related more to cell shape than to cell proliferation.
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PMID:Effect of cell shape on proteinase secretion by epithelial cells. 282 Oct 27

A 5.25-year-old male Yorkshire Terrier was evaluated for suspected thrombosis of the distal portion of the aorta following protein-losing enteropathy. Hind limb paralysis was evident, extremities were hypothermic, and femoral pulses were not palpable. A thrombus was found in the distal portion of the aorta using Doppler ultrasonography. Enteropathy-induced loss of albumin and antithrombin III was the suspected cause of hypercoagulability in this dog. Alteplase, a recombinant tissue-plasminogen activator, was used to recanalize the distal portion of the aorta without inducing clinically evident systemic fibrinogenolysis. Alteplase is a fibrin-specific activator of plasminogen and may be safer and more efficacious than conventional streptokinase treatment. Current information on use in thromboembolic disease in human beings suggests that administration of alteplase as a bolus leads to earlier arterial patency and reduced risk of hemorrhage.
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PMID:Use of recombinant tissue-plasminogen activator for aortic thrombolysis in a hypoproteinemic dog. 949 Nov 62

The poly(D,L-lactic acid)-block-(ligand-tethered poly(ethylene glycol)) copolymer was explored to engineer poly(D,L-lactic acid) (PLA) material to promote chondrocyte attachment and growth. The poly(D,L-lactic acid)-block-poly(ethylene glycol) copolymer (PLE) was synthesized by a coupling reaction between PLA and poly(ethylene glycol) (PEG) (M(n) 1000, 2000, and 4000 respectively), with the use of 4,4'-methylenediphenyl diisocyanate (MDI). Then the PLE was activated by methyl sulfonyl chloride and the amino acids or arginine-glycine-aspartic acid tripeptide (RGD) was attached, which was verified by the ninhydrin-UV method. The modified PLA films were simply prepared by blending PLA with PLE derivatives. ATR-FTIR, XPS, contact angle, and AFM results clearly showed that the PEG chain stably enriched on the surface of PLE-modified PLA films. The chondrocyte cytocompatibility test showed the modified PLA films could significantly improve chondrocyte attachment and proliferation.
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PMID:Poly(D,L-lactic acid)-block-(ligand-tethered poly(ethylene glycol)) copolymers as surface additives for promoting chondrocyte attachment and growth. 1613 Jan 43

A quantitative trait loci (QTL) analysis for androgenetic capability has been conducted on three different crosses in maize, including very high and nonresponding lines for androgenesis. The doubled haploid lines derived by anther culture from the crosses DH5 x DH7, A188 x DH7, and R6 x DH99 showed a range of 0-70%, 0-40%, and 0-50% androgenetic responding anthers, respectively. The genotypic heritability of means for this trait is close to 0.90 for A188 x DH7 and 0.78 for R6 x DH99. The QTL analysis involved in each population the mapping of more than 100 loci covering a large part of the genome with reasonably spaced markers averaging 12 cM. Different measurements describing the androgenetic process were studied: AC, percentage of responding anthers; ELS, number of androgenetic embryos produced per 100 plated anthers; PLE, number of plantlets regenerated per 100 embryos; PLA, number of plantlets per 100 plated anthers. In each cross, three to four QTLs were found for AC, explaining 30-40% of the phenotypic variation. The QTL detected for PLA was also strong QTL for AC or ELS. This agrees with the observation that these last two traits are good predictors for final plantlet yield. The QTLs found were specific, although the same line DH7 was used in two crosses and DH99 derived from DH5 and DH7 in the third cross. These results suggest that the transfer of the androgenetic capabilities in elite germplasm will still involve a phenotypic evaluation of the androgenetic performances. A backcross-assisted selection based only on the genotype at the QTL is probably possible but only within the crosses used for this QTL analysis.
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PMID:Genotypic variation of quantitative trait loci controlling in vitro androgenesis in maize. 1847 Jan 36

In our previous work, a new kind of pH-sensitive hydrogel based on poly (lactic acid) (PLA), methoxyl poly (ethylene glycol) (MPEG) and itaconic acid (IA) was prepared by heat-initiated free radical polymerization without any organic solvent. For further application, the safety of prepared copolymers and hydrogels was evaluated in vitro and in vivo in this paper. In vitro toxicity evaluation of MPEG-PLA-AC (PLE-AC) copolymers included hemolytic test and cytotoxicity which were evaluated by cell viability assay using HEK293 cells. In vivo acute toxicity tests and histopathological study of PLE-AC copolymers were carried out on BALB/c mice which were administrated copolymers (1 g/kg b.w.) intravenously. In acute toxicity test, the mice were observed continuously for 7 days, their body weight obtained everyday, at last the mice were sacrificed for the following study: the blood of the mice was assigned for blood chemistry and routine analysis, the heart, liver, spleen, lung, and kidneys were used for histopathological study. In acute oral toxicity test, mice were orally administered with a total 15 g/kg body weight (b.w.) of P(LE-IA-MEG) hydrogels, and were observed continuously for 14 days. For histopathologic study, samples including heart, liver, lung, kidneys, spleen, stomach, and intestine, were histochemically prepared and stained with hematoxylin-eosin for histopathologic examination. All results indicated that the PLE-AC copolymers and pH-sensitive hydrogels were non-toxic. Therefore it might be used as a safe candidate for drug delivery system.
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PMID:Preliminary assessment of the safety evaluation of novel pH-sensitive hydrogel. 2288 57