Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
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Gene/Protein
Disease
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Target Concepts:
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Query: UNIPROT:O76050 (
neu
)
3,969
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Serum-free mouse embryo (SFME) cells are a cell line derived in medium in which serum is replaced with growth factors and other supplements. These cells display unusual properties: a) they do not lose proliferative potential or show gross chromosomal aberration upon extended culture, b) they depend on epidermal growth factor (EGF) for survival, and c) they are reversibly growth inhibited by plasma and serum. Transfection of SFME cells with oncogenes (ras,
neu
, SV40 T antigen) results in cells that grow in serum-supplemented medium and no longer require EGF for survival. The growth inhibitory activity of human plasma on SFME cells was investigated. The activity was present in delipidated plasma and was not dialyzable against 1 M
acetic acid
. The activity precipitated in 33% methanol, bound to concanavalin A-agarose and was retarded by Sephadex G-50 in 200 mM
acetic acid
. A fifty- to one-hundred-fold purification was achieved, although most of the differential inhibition of untransformed vs. transformed cells was lost in the course of the purification.
...
PMID:Characterization of human plasma growth inhibitory activity on serum-free mouse embryo cells. 768 44
Serum-free mouse embryo (SFME) cells are a cell line derived in medium in which serum is replaced with growth factors and other supplements. These cells display unusual properties: a) they do not lose proliferative potential or show gross chromosomal aberration upon extended culture, b) they depend on epidermal growth factor (EGF) for survival, and c) they are reversibly growth inhibited by plasma and serum. Transfection of SFME cells with oncogenes (ras,
neu
, SV40 T antigen) results in cells that grow in serum-supplemented medium and no longer require EGF for survival. The growth inhibitory activity of human plasma on SFME cells was investigated. The activity was present in delipidated plasma and was not dialyzable against 1M
acetic acid
. The activity precipitated in 33% methanol, bound to concanavalin A-agarose and was retarded by Sephadex G-50 in 200 mM
acetic acid
. A fifty- to one-hundred-fold purification was achieved, although most of the differential inhibition of untransformed vs. transformed cells was lost in the course of the purification.
...
PMID:Characterization of human plasma growth inhibitory activity on serum-free mouse embryo cells. 2751 54
The effects of aqueous extract of
Schizandra Chinensis Fruit
(AESC) on cadmium-induced changes of monoamine neurotransmitters in the different brain regions of adult rats were investigated. Male rats were received intraperitoneal (i.p.) administration of CdCl
2
(0.6 mg/kg/d) for 21 days and sacrificed 7 days after the last administration. Concentrations of norepinephrine (NE), dopamine (DA) in striatum and serotonin (5-HT), 5-hydroxyindole
acetic acid
(5-HIAA) in cortex were measured by HPLC. There were significant decreases of NE, DA, 5-HT and 5-HIAA in Cd intoxicated rats (
P
< 0.05), while pretreatment with AESC (20 mg/kg/d or 60 mg/kg/d, p.o., 30 min before CdCl
2
) greatly inhibited the decrease of monoamine transmitters, respectively (
P
< 0.05). Also, AESC significantly increased the reduction of glutathione contents and superoxide dismutase activities in cortex induced by CdCl
2
. These results suggest that AESC ameliorates Cd-induced depletion of monoamine
neu
-rotransmitters in brain through its antioxidant activity.
...
PMID:Effects of Aqueous Extract of
Schizandra Chinensis Fruit
on Cadmium-Induced Change of Monoamine Neurotransmitters in Rats. 3203 14
