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Query: UNIPROT:O75191 (H. influenzae)
 4,961 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Twelve strains of Haemophilus ducreyi isolated primarily from chancroid outbreaks in North America were examined for the presence of pili by transmission electron microscopy. We identified piliated cells in 10 of the 12 strains. Pilin extracts were prepared from the mechanically sheared cells of the 12 H. ducreyi strains as well as the stably piliated H. influenzae strain R890 and its non-piliated parent R906. Pili were present in 12 out of 12 H. ducreyi extracts and in the R890 extract but not in the R906 preparation. Pili were purified by cycles of differential pH solubilization and crystallization. In SDS-PAGE, the preparation consisted predominantly of a protein whose apparent relative molecular mass was 24,000 (24 k), and an electron micrograph showed that the preparation contained pili. Three H. ducreyi strains were passed 52 times on agar plates, and extracts prepared from these strains contained pili. There was no evidence of binding of erythrocytes obtained from nine mammalian and avian species to colonies of one of the stably piliated H. ducreyi strains. We conclude that H. ducreyi expressed pili, that the relative molecular mass of the pilin monomer was 24 k, that pilus expression was not readily lost in passage and that H. ducreyi pili may not bind to an erythrocyte receptor.
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PMID:Characterization of pili expressed by Haemophilus ducreyi. 198 53

Inhibition of adherence of bacteria to epithelial cells contributes to a reduction of infections by these bacteria. We have shown that the Anton blood group antigen, the erythrocyte receptor for Haemophilus influenzae (van Alphen et al. 1986, FEMS Microbiol. Lett. 37, 69-71), occurs in saliva, that the occurrence is not related to the secretor state of the donor of the saliva and that saliva containing Anton antigen could not inhibit the adherence of H. influenzae to oropharynx epithelial cells. Anton antigen was detected in saliva samples of 14 donors by immunoblotting with two different anti-Anton sera. The amount of Anton antigen correlated with the ability of H. influenzae to adhere to the epithelial cells of the donor of the saliva: 4.1 +/- 0.1 Anton antigen units for donors with more than 50 H. influenzae per cell and 1.6 +/- 0.5 units for donors with less adhering epithelial cells. No correlation between the amount of Anton antigen in saliva and secretor status of the donor was observed. Adherence of H. influenzae to epithelial cells was not inhibited by saliva of secretors (N = 11) or non-secretors (N = 3). The same saliva did not inhibit the interaction of the bacteria with Anton antigen bearing erythrocytes as measured by haemagglutination inhibition. This indicates that the amount of Anton antigen in saliva is probably too low to interfere with the interaction of H. influenzae with oropharynx epithelial cells and erythrocytes.
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PMID:Relationship between secretion of the Anton blood group antigen in saliva and adherence of Haemophilus influenzae to oropharynx epithelial cells. 263 75

The Anton blood group antigen has been shown to be the erythrocyte receptor for Haemophilus influenzae. Cord erythrocytes, which lack the Anton antigen, were not agglutinated by H. influenzae (L. van Alphen, J. Poole, and M. Overbecke, FEMS Microbiol. Lett. 37:69-71, 1986). Twenty-eight erythrocyte suspensions from newborns less than 4 days old were also not agglutinated, but 23 of 56 erythrocyte suspensions from 4- to 50-day-old newborns and 23 of 35 erythrocyte suspensions from older infants were agglutinated. Positive hemagglutination correlated with the presence of the Anton antigen on the erythrocytes for 163 of 173 (P less than 0.0001). Adherence of H. influenzae to buccal epithelial cells obtained from six newborns within 3 days after birth was as strong as that found with adult epithelial cells, whereas the erythrocytes from five of six of these newborns were not agglutinated by the bacteria. Adherence of H. influenzae to epithelial cells of 15 donors was not inhibited by anti-Anton serum. Moreover, H. influenzae carrying fimbriae adhered to epithelial cells of an Anton-negative donor. From these results we conclude that the age at which the erythrocyte receptor for H. influenzae is expressed is the same as for the Anton antigen, but that the receptor on the epithelial cells is already expressed at birth and is not identical to the Anton antigen.
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PMID:The erythrocyte and epithelial cell receptors for Haemophilus influenzae are expressed independently. 288 31

In this study we isolated the pilin gene from the Brazilian purpuric fever (BPF) clone of Haemophilus influenzae biogroup aegyptius, expressed the gene in Escherichia coli, and determined its nucleotide sequence. Comparison of the nucleotide sequence of the BPF pilin gene with the sequences of pilin genes from strains of H. influenzae sensu stricto demonstrated a high degree of identity. Consistent with this observation, hemagglutination inhibition studies performed with a series of glycoconjugates indicated that BPF pili and H. influenzae type b pili possess the same erythrocyte receptor specificity.
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PMID:Isolation, expression, and nucleotide sequencing of the pilin structural gene of the Brazilian purpuric fever clone of Haemophilus influenzae biogroup aegyptius. 847 16