Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:B0FTZ7 (
catenin
)
18,795
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Multi-PDZ (PSD-95/Discs large/Zonula-occludens-1) domain proteins play a crucial role in the establishment and maintenance of cell polarization. The novel multi-PDZ domain protein
FRMPD2
is a potential scaffolding protein consisting of an N-terminal KIND domain, a FERM domain and three PDZ domains. Here we show that
FRMPD2
is localized in a polarized fashion in epithelial cells at the basolateral membrane and partially colocalizes with the tight-junction marker protein Zonula-occludens-1. Downregulation of
FRMPD2
protein in Caco-2 cells is associated with an impairment of tight junction formation. We find that the FERM domain of
FRMPD2
binds phosphatidylinositols and is sufficient for membrane localization. Moreover, we demonstrate that recruitment of
FRMPD2
to cell-cell junctions is strictly E-cadherin-dependent, which is in line with our identification of
catenin
family proteins as binding partners for
FRMPD2
. We demonstrate that the FERM domain and binding of the PDZ2 domain to the armadillo protein p0071 are required for basolateral restriction of
FRMPD2
. Moreover, the PDZ2 domain of
FRMPD2
is sufficient to partially redirect an apically localized protein to the basolateral membrane. Our results provide novel insights into the molecular function of
FRMPD2
and into the targeting mechanism of peripheral membrane proteins in polarized epithelial cells.
...
PMID:PDZ-domain-directed basolateral targeting of the peripheral membrane protein FRMPD2 in epithelial cells. 1970 87
