Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UNIPROT:A7BJS8 (
F-box and WD40 domain protein 7
)
5
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
FBXW7 (
F-box and WD40 domain protein 7
) is a tumor suppressor frequently inactivated in human cancers. The precise molecular mechanisms by which FBXW7 exerts antitumor activity remain under intensive investigation and are thought to relate in part to FBXW7-mediated destruction of key cancer-relevant proteins.
Enolase 1
(
ENO1
) possesses oncogenic activity and is often overexpressed in various human cancers, besides its critical role in glycolysis. However, the detailed regulatory mechanisms of
ENO1
expression remain unclear. Here we show that the elevated expression of
ENO1
was identified in FBXW7-depletion HCT116 cells through two-dimensional protein electrophoresis and mass spectrometry assays (2DE-MS). Subsequent western blotting and immunohistochemical assays confirmed that
ENO1
expression reversely correlates with FBXW7 expression in several cells and colon cancer tissues. Furthermore, we show that FBXW7 physically binds to
ENO1
and targets
ENO1
for ubiquitin-mediated degradation. Functionally, we found that FBXW7 suppresses the
ENO1
-induced gene expression, lactate production, cell proliferation and migration. These findings suggest that
ENO1
is a novel substrate of FBXW7, and its activity can be negatively regulated by FBXW7 at the posttranslational level. Our work provides a novel molecular insight into FBXW7-directed tumor suppression through regulation of
ENO1
.
...
PMID:FBXW7 negatively regulates ENO1 expression and function in colorectal cancer. 2609 98
