Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C1864663 (
HCC
)
2,985
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
AIM:We have previously reported that inducible over-expression of Bak may prolong cell cycle in G(1) phase and lead to apoptosis in
HCC
-9204 cells. This study is to investigate whether
p27(KIP1)
plays an important role in this process.METHODS:In order to elucidate the exact function of
p27(KIP1)
in this process, a zinc inducible
p27(KIP1)
stable transfectant and transient
p27(KIP1)
-GFP fusion transfectant were constructed. The effects of inducible
p27(KIP1)
on cell growth, cell cycle arrest and apoptosis were examined in the mock, control pMD vector, and pMD-KIP1 transfected
HCC
-9204 cells.RESULTS:This
p27(KIP1)
-GFP transfectant may transiently express the fusion gene. The cell growth was reduced by 35% at 48 h of
p27(KIP1)
induction with zinc treatment as determined by trypan blue exclusion assay. These differences remained the same after 72h of
p27(KIP1)
expression.
p27(KIP1)
caused cell cycle arrest after 24 h of induction, with 40% increase in G(1) population. Prolonged
p27(KIP1)
expression in this cell line induced apoptotic cell death reflected by TUNEL assay. Fourty-eight h and 72 h of
p27(KIP1)
expression showed a characteristic DNA ladder on agarose gel electrophoresis.CONCLUSION:Bak may induce cell cycle arrest in G(1) phase through upregulating expression of
p27(KIP1)
and subsequently lead to apoptosis in
HCC
-9204 cells. The
p27(KIP1)
-GFP fusion protein can be transiently expre-ssed in
HCC
-9204 cells. The inducible
p27(KIP1)
-expressing cell line provides a model to assess
p27(KIP1)
function.
...
PMID:Overexpression of p27(KIP1) induced cell cycle arrest in G(1) phase and subsequent apoptosis in HCC-9204 cell line. 1181 39
