Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C1864663 (
HCC
)
2,985
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Exonuclease 1
(
EXO1
), a member of the RAD2 nuclease family, was first described as possessing 5' to 3' nuclease activity and 5' structure-specific endonuclease activity. Here, we show that
EXO1
is significantly upregulated in
HCC
tumor tissues and that high
EXO1
expression is significantly correlated with liver cirrhosis. We further demonstrate that
EXO1
knockdown decreases proliferation and colony forming abilities of
HCC
cells
in vitro
and tumorigenicity
in vivo
, as well as decreases migration and invasive capabilities of
HCC
cells. Alternatively,
EXO1
overexpression significantly increases the proliferation, colony forming ability, and migration and invasive capabilities of
HCC
cells
in vitro
. Additionally, we truncated a region upstream of the transcription start site (TSS) of
EXO1
and used the region with the strongest transcriptional activity to predict that the transcription factor
FOXP3
can bind to the
EXO1
promoter. Bioinformatics analysis found that
FOXP3
was positively correlated with
EXO1
and luciferase reporter assays and RT-PCR confirmed that
FOXP3
could enhance the transcriptional activity of
EXO1
. CCK-8 assays showed that depletion of
FOXP3
further reduces cell proliferation ability after knocking down of
EXO1
in vitro
. Taken together, our findings indicate that
EXO1
acts as an oncogene in
HCC
and its expression level is related to
FOXP3
activity.
...
PMID:
EXO1
Plays a Carcinogenic Role in Hepatocellular Carcinoma and is related to the regulation of
FOXP3
. 3262 39
