Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C1862200 (RHE)
 1,093 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Simian retroviruses (SRVs), the etiological agent of a spontaneous Simian acquired immunodeficiency syndrome, endemically infects large percentages of Asian macaques housed in biomedical research colonies and severely compromises the effective use of these species as a viable research animal. We recently described the molecular cloning of a serogroup 2 SRV, D2/RHE/OR, which causes mild immunosuppression in rhesus macaques. A restriction site variant, D2/RHE/OR/V1, has also been recovered from severely ill animals endemically infected with D2/RHE/OR. We now report the complete nucleotide sequences of D2/RHE/OR and D2/RHE/OR/V1. Both infectious molecular clones retain the genetic structure typical of type D SRVs (5' LTR-gag-prt-pol-env-3'LTR) and encode identically sized 8105-bp proviruses. D2/RHE/OR and D2/RHE/OR/V1 are 99.3% similar at the amino acid level, exhibiting only 17 residue differences, of which 10 are located in the envelope glycoproteins. The molecular clones and reciprocal chimeric viruses were used to assess the contribution of different genetic domains to virus infectivity in a T cell infection assay. These experiments indicate that D2/RHE/OR has a reduced ability to infect specific T cell lines, especially Hut-78 and MT-4 cells, and that the envelope gene is not the sole determinant of in vitro tropism.
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PMID:Molecular cloning and cell-specific growth characterization of polymorphic variants of type D serogroup 2 simian retroviruses. 1044 55

The simian type D retroviruses (SRVs) are one of the causative agents of simian acquired immunodeficiency syndrome (SAIDS) in Asian macaques. In this report, we describe the infection of a rhesus macaque with the SRV serogroup 5 isolate, D5/RHE/OR. Using reverse transcriptase-polymerase chain reaction (RT-PCR) and dot blot hybridization analyses, we have determined the tissue distribution of D5/RHE/OR in this infected rhesus macaque, and have demonstrated viral mRNA in the majority of the surveyed tissues, including robust loads in the bone marrow, seminal vesicle, submaxillary salivary gland, prostate, and skeletal muscle. Microscopic examination of necropsy tissues revealed generalized lymphoid hyperplasia that was most severe in the salivary gland, bone marrow, kidney, and spleen. We also describe the first sequence analyses of portions of the D5/RHE/OR gag-prt region, obtained as a RT-PCR amplification product from infected rhesus macaque tissue, and report the first confirmation using Northern blot analyses that the SRV serogroups, including D5/RHE/OR, express similarly-sized genomic and subgenomic env mRNAs.
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PMID:Simian retrovirus serogroup 5: partial gag-prt sequence and viral RNA distribution in an infected rhesus macaque. 1112 42

We have recently identified and sequenced a molecular clone of the serogroup 2 simian retrovirus (SRV), D2/RHE/OR/V1, that retains an enhanced ability to infect specific T cell lines. In this report, using deletion mutagenesis, we localized the psi packaging signal, necessary for packaging of D2/RHE/OR/V1 particles, to the genomic region 345-650, which comprises the 5' intergenic region (IR) and the extreme 5' portion of the gag gene. To build an SRV-based gene transfer system and to reduce the possibility of recombination and regeneration of replication-competent viruses, we constructed split-genome D2/RHE/OR/V1 plasmid recombinants containing distinct and non-overlapping retroviral gene regions and several replacement components. For the retrovirus gene transfer vehicle, we deleted the D2/RHE/OR/V1 structural genes and substituted a cassette including the psi-packaging region, the beta-galactosidase reporter gene, and the 3' IR. Both packaging cell recombinants were used to generate stable monkey packaging cell lines; the gene transfer vehicle was subsequently transfected into the packaging cell lines, and replication-defective viruses were recovered for subsequent infection into fresh monkey cells. Successful infection by the recovered viruses verifies the potential efficacy of the SRV-based system as a research tool for gene transfer of heterologous genes into nonhuman primate cells.
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PMID:Simian retrovirus vectors for gene transfer in nonhuman primate cells. 1132 70