Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C1855645 (
KPC
)
1,473
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Objectives:
Current worldwide spread of carbapenem resistance in Enterobacteriaceae constitutes a critical public health threat. This study aims to investigate how carbapenem resistance is acquired in Enterobacteriaceae in patients during antimicrobial therapy.
Methods:
Clinical strains from the same anatomical site of the same patients that converted from carbapenem-susceptible to resistant during antimicrobial therapy and showed identical or similar PFGE patterns were identified. The similarly sized plasmids carried by the susceptible and resistant strains, the latter containing the carbapenemase genes, were sequenced and analyzed.
Results:
Paired strains were identified from four patients: three had neurosurgical conditions while the other had acute exacerbation of
COPD
. Two pairs of
Klebsiella pneumoniae
(KP1-S/R and KP2-S/R, S and R indicating susceptible and resistant strains, respectively), one pair of
Morganella morganii
(MM-S/R) and one pair of
Enterobacter aerogenes
(EA-S/R) were collected. All four carbapenem-resistant strains carried plasmids harboring
bla
KPC
-2
. Compared with the similarly sized plasmids in KP1-S and KP2-S, an insertion sequence that includes IS
Kpn6
-like,
bla
KPC
-2
and IS
Kpn8
was noted in pKP1-R and pKP2-R. Strains MM-R and EA-R had
bla
KPC
-2
-carrying plasmids not resembling plasmids in strains MM-S and EA-S suggesting their new acquisition while on therapy.
Conclusions:
Enterobacteriaceae can acquire carbapenem resistance during antimicrobial therapy through horizontal transfer of an insertion sequence or plasmid.
...
PMID:
In vivo
Acquisition of Carbapenemase Gene
bla
KPC-2
in Multiple Species of
Enterobacteriaceae
through Horizontal Transfer of Insertion Sequence or Plasmid. 2781 49
