Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C1855645 (
KPC
)
1,473
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Beta-lactam resistant bacteria, which are commonly resident in tertiary hospitals, have emerged as a worldwide health problem because of ready-to-eat vegetable intake. We aimed to characterize the genes that provide resistance to beta-lactam antibiotics in Enterobacteriaceae, isolated from five commercial salad brands for human consumption in Mexico City. In total, twenty-five samples were collected, grown in blood agar plates, and the bacteria were biochemistry identified and antimicrobial susceptibility testing was done. The carried family genes were identified by endpoint PCR and the specific genes were confirmed with whole genome sequencing (WGS) by Next Generation Sequencing (NGS). Twelve positive cultures were identified and their microbiological distribution was as follows: 8.3% for
Enterobacter aerogene
(
n
= 1), 8.3% for
Serratia fonticola
(
n
= 1), 16.7% for
Serratia marcesens
(
n
= 2), 16.7% for
Klebsiella pneumoniae
(
n
= 2), and 50% (
n
= 6) for
Enterobacter cloacae
. The endpoint PCR results showed 11 colonies positive for
bla
BIL (91.7%), 11 for
bla
SHV (91.7%), 11 for
bla
CTX (97.7%), 12 for
bla
DHA (100%), four for
bla
VIM (33.3%), two for
bla
OXA (16.7%), two for
bla
IMP (16.7%), one for
bla
KPC
(8.3%), and one for
bla
TEM (8.3%) gen; all samples were negative for
bla
ROB,
bla
CMY,
bla
P,
bla
CFX
and
bla
LAP gene. The sequencing analysis revealed a specific genotype for
Enterobacter cloacae
(blaSHV-12, blaCTX-M-15, blaDHA-1, blaKPC-2);
Serratia marcescens
(blaSHV-1, blaCTX-M-3, blaDHA-1, blaVIM-2);
Klebsiella pneumoniae
(blaSHV-12, blaCTX-M-15, blaDHA-1);
Serratia fonticola
(blaSHV-12, blaVIM-1, blaDHA-1); and,
Enterobacter aerogene
(blaSHV-1, blaCTX-M-1, blaDHA-1, blaVIM-2, blaOXA-9). Our results indicate that beta-lactam-resistant bacteria have acquired integrons with a different number of genes that provide pan-resistance to beta-lactam antibiotics, including penicillins, oxacillins, cefalosporins, monobactams, carbapenems, and imipenems.
...
PMID:Characterization of Gene Families Encoding Beta-Lactamases of Gram-Negative Rods Isolated from Ready-to-Eat Vegetables in Mexico City. 3047 53
