Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C1852044 (HS3)
 171 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

PHA-stimulated human lymphocytes contain the protein (SBP) which has selectivity in binding of 1.8 kb fragment of human satellite DNA III (HS3) as compared to other DNA sequences. It is shown that the binding site is localized within 1kb Sau3A-EcoR I fragment of HS3. SBP-binding activity is increased after treatment of cells with tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA). The essential increase in a number of metaphases with chromosome endoreduplications in TPA-treated lymphocytes indicates that SBP may be involved in initiation of chromosome replication or in alteration of the mitotic spindle function.
Mol Gen Mikrobiol Virusol 1988 Apr
PMID:[Presence in human lymphocytes of a protein specifically binding to a cloned fragment of human satellite DNA III]. 284 95

In nuclear extract of HeLa cells two proteins were identified having the specific binding activity to cloned 1.8kb fragment of human satellite DNA III (HS3). One of the satellite binding proteins (SBP1) purified by column chromatography using DEAE-, phospho- and DNA-cellulose steps interacted also with adenovirus 5 replication enhancer (ARE), another protein (SBP2) was separated during phosphocellulose chromatography from ARE-binding protein. It is suggested that SBP1 is possibly identical to the nuclear factor I purified earlier from the nuclear extract of HeLa cells by other authors.
Mol Gen Mikrobiol Virusol 1988 Apr
PMID:[Purification and properties of a protein from the nuclear fraction of HeLa cells binding to a cloned fragment of human satellite DNA III]. 340 31

Fusion of the Chinese hamster stably transformed Tk+ cells containing chromosomally integrated plasmid with a fragment of human satellite III DNA (HS3), with mitomycin C-treated human cells (B-lymphoma, line Raji) results in amplification and excision of heterogeneous plasmid material from hamster chromosomes. Some of these plasmids contain HS3. Functional activity of HS3 in the initiation of DNA replication is shown in transient transfection experiments. The results indicate that mitomycin C induces in human cells some trans-acting factors (possibly proteins) activating a replication origin within HS3 DNA and leading to plasmid replication in situ and their excision from chromosomes.
Mol Gen Mikrobiol Virusol 1987 Jun
PMID:[Excision of the plasmid containing the inserted human satellite DNA from Chinese hamster chromosomes after fusion of hamster and human cells]. 362 35