Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C1852044 (
HS3
)
171
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The distal locus control region (LCR) is required for high-level expression of the complex of genes (HBBC) encoding the beta-like globins of mammals in erythroid cells. Several major DNase hypersensitive sites (HSs 1-5) mark the LCR. Sequence conservation and direct experimental evidence have implicated sequences within and between the HS cores in function of the LCR. In this report we confirm the mapping of a minor HS between
HS3
and HS4, called
HS3
.2, and show that sequences including it increase the number of random integration sites at which a drug resistance gene is expressed. We also show that nuclear proteins including
GATA1
and Oct1 bind specifically to sequences within
HS3
.2. However, the protein Pbx1, whose binding site is the best match to one highly conserved sequence, does not bind strongly.
GATA1
and Oct1 also bind in the HS cores of the LCR and to promoters in HBBC. Their binding to this minor HS suggests that they may be used in assembly of a large complex containing multiple regulatory sequences.
...
PMID:Functional and binding studies of HS3.2 of the beta-globin locus control region. 1186 25
