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Query: UMLS:C1832588 (PSS)
 2,979 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Interactions between surfaces bearing multilayer films of poly(allylamine hydrochloride) (PAH) and poly(styrenesulfonate sodium salt) (PSS) were investigated across a range of aqueous KBr solutions. Three layer films (PAH/PSS/PAH) were preassembled on mica surfaces, and the resulting interactions were measured with the interferometric surface force apparatus (SFA). Increasing the ionic strength of the medium resulted in a progressive swelling of the multilayer films. Interactions in solutions containing more than 10(-3) M KBr were dominated by a long-ranged steric repulsion originating from compression of polyelectrolyte segments extending into solution. In 10(-1) M KBr, repeated measurements at the same contact position showed a considerable reduction of the range and the strength of the steric force, indicating a flattening of the film during initial approach. Furthermore, this flattening was irreversible on the time scale of the experiments, and measurements performed up to 72 h after the initial compression showed no signs of relaxation. These studies aid in understanding the dominant interactions between polyelectrolyte multilayers, including polyelectrolyte films deposited on colloidal particles, which is important for the preparation of colloidally stable nanoengineered particles.
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PMID:Surface interactions during polyelectrolyte multilayer build-up. 2. The effect of ionic strength on the structure of preformed multilayers. 1661 58

Poly(L-lactide) (PLLA) surface was modified via aminolysis by poly(allylamine hydrochloride) (PAH) at high pH and subsequent electrostatic self-assembly of poly(sodium styrenesulfonate) (PSS) and PAH, and the process was monitored by X-ray photoelectron spectroscopy (XPS) and contact angle measurement. These modified PLLAs were then used as charged substrates for further incorporation of gelatin to improve their cytocompatibility. The amphoteric nature of the gelatin was exploited and the gelatin was adsorbed to the negatively charged PLLA/PSS and positively charged PLLA/PAH at pH=3.4 and 7.4, respectively. XPS and water contact angle data indicated that the gelatin adsorption at pH=3.4 resulted in much higher surface coverage by gelatin than at pH=7.4. All the modified PLLA surfaces became more hydrophilic than the virgin PLLA. Chondrocyte culture was used to test the cell attachment, cell morphology and cell viability on the modified PLLA substrates. The results showed that the PAH and PSS modified PLLA exhibited better cytocompatibility than virgin PLLA, and the incorporation of the gelatin on these modified PLLA substrates further improved their cytocompatibility, with the PLLA/PSS substrate treated with the gelatin at pH=3.4 being the best, exceeding the chondrocyte compatibility of the tissue culture polystyrene.
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PMID:Surface modification of poly(L-lactic acid) to improve its cytocompatibility via assembly of polyelectrolytes and gelatin. 1670 73

When neuron-like cells (NLCs) derived from pluripotent embryonal carcinoma cells (P19) are cultured on bare tissue culture substrates, they require a monolayer of fibroblast cells to exhibit normal neurite outgrowth, behavior typical of neuronal cultures. However, substrate treatment with polyelectrolyte multilayers (PEMs) composed of poly(allylamine hydrochloride) (PAH) and poly(styrenesulfonic acid) (PSS) significantly improved these cultures. Cell morphology was more spread, indicative of healthy cells, and direct attachment of neuronal cell bodies to the treated surface was observed. Neuronal outgrowth across the surface was not dependent on an underlying fibroblast monolayer with the PEMs surface treatment. Additionally, the PEMs surface treatment can be used to condition various surfaces, facilitating neuronal cultures on surfaces which are natively hydrophilic (tissue culture polystyrene) or hydrophobic (poly(dimethylsiloxane), PDMS). Microfluidic networks were used to micropattern the PEMs onto PDMS, resulting in confined regions of cellular attachment and directed neuronal outgrowth. The ability of PEMs to encourage NLC attachment without supporting cells to a variety of surfaces and surface geometries greatly simplifies neuronal culture methodology and enables neuronal investigations in new environments.
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PMID:Facilitating the culture of mammalian nerve cells with polyelectrolyte multilayers. 1676 7

Neutron reflectivity measurements were used to investigate the thermal stability of layer-by-layer (LBL) films of poly(o-methoxyaniline) (POMA), which was probed by increasing the temperature up to 80 degrees C of a D2O solution in contact with the LBL films. The study was made possible by adsorbing POMA layers on a PEI/(PSS/PAH)5/PSS LBL film template, leading to less rough POMA layers in comparison with the POMA/poly(vinylsulfonic acid sodium salt) (PVS) LBL films adsorbed directly on glass and silicon substrates. While the latter yielded almost fringeless neutron reflectivity curves due to the large roughness, the fitting of the data for POMA films adsorbed onto the template film and UV-vis measurements indicated that the topmost layer is affected for films heated in solution up to 80 degrees C. This is essentially the same thermal stability of LBL films from the template films made with conventional polyelectrolytes. A decrease in thickness of approximately 10 A was inferred when the solution temperature increased from 25 degrees C to 80 degrees C, which was maintained when the sample was cooled back to 25 degrees C. This decrease, observed for solutions of pH 3 and pH 8, is consistent with thermally-stimulated desorption and was corroborated by UV-VIS absorption experiments. The unexpected stability of the POMA layer at pH 8 is attributed to the layer-by-layer structure of the films that allows POMA to remain doped, in its salt emeraldine form, even at high pH.
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PMID:Thermal stability of poly(o-methoxyaniline) layer-by-layer films investigated by neutron reflectivity and UV-VIS spectroscopy. 1679 71

Interpolyelectrolyte complex (IPEC) formation between poly(styrene sulfonate) (PSS) and poly(allylamine hydrochloride) (PAH) has been studied over a range of ionic strengths by isothermal titration calorimetry (ITC), turbidity titration, and electrostatic layer-by-layer assembly (ELBL). The results indicate that IPEC formation of PSS/PAH in aqueous solution is predominantly entropy-driven. The thermodynamic parameters suggest the formation of different types of complexes and aggregates due to salt-induced conformational changes in the polyelectrolyte conformation. Differences in polyelectrolyte behavior in the different salt-concentration regimes are described in terms of changes in the Debye screening length of the polyelectrolytes. The relationship of the results to the effect of salt concentration on the assembly of polyelectrolyte multilayer films (PEMs) is discussed.
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PMID:Direct determination of the thermodynamics of polyelectrolyte complexation and implications thereof for electrostatic layer-by-layer assembly of multilayer films. 1680 Jun 64

pH-induced hysteretic gating of track-etched polycarbonate membranes (TEPC) has been achieved by depositing layer-by-layer assembled polyelectrolyte multilayers comprising poly(allylamine hydrochloride) (PAH) and poly(sodium 4-styrenesulfonate) (PSS) at a high pH condition (pH > 9.0). Scanning electron microscopy and transmission electron microscopy showed that the average bilayer thickness of multilayers was greater within the cylindrical pores of track-etched polycarbonate membranes compared to the multilayers on planar substrates (e.g., Si wafers and the face of TEPC membranes). Swelling/deswelling properties of multilayers and gating properties of the multilayer-modified TEPC membranes were studied by measuring the flux of pH-adjusted deionized water. Large discontinuous changes in the transmembrane flux were observed, indicating that the multilayers within the cylindrical pores of TEPC membranes exhibit the discontinuous swelling/deswelling behavior observed previously for planar systems. The degree of swelling as estimated by simple models, however, showed that (PAH/PSS) multilayers in the confined geometry swelled to smaller extents compared to the same multilayers on planar substrates under the same conditions. Multilayer-modified membranes showed reversible gating properties as the pH condition of feed solution was alternated between pH 2.5 and 10.5. In situ atomic force microscopy (AFM) was used to visualize the closing of the pores as a function of time. The hysteretic gating property of the multilayer-modified TEPC membrane was utilized to achieve either a "closed" or "open" state at one pH condition depending on the pretreatment history, thereby enabling either the retention or passage of high-molecular weight polymers by varying the membrane pretreatment condition.
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PMID:pH-induced hysteretic gating of track-etched polycarbonate membranes: swelling/deswelling behavior of polyelectrolyte multilayers in confined geometry. 1680 18

Decellularized allograft tissues have been identified as a potential extracellular matrix scaffold for tissue-engineered vascular substitutes. In order to improve the thromboresistance, it is necessary to pre-coat the intra-luminal vessel surface. Recently a new surface modification technique appeared, based on the alternate adsorption of positive and negative charged polyelectrolytes. Our objective was to develop an alternative vascular scaffold made of decellularized human umbilical arteries treated with a PAH/PSS polyelectrolyte multilayered film. The vessels luminal surfaces covered with the multilayer film were observed by electronic scanning microscopy. Our observations showed that the luminal surface is completely devoid of ECs following treatment with trypsin. A top view of the coated artery indicated that the multilayer uniformly covered internal surface of the vessels. The successful of the multilayer correct deposition and retention on the arterial wall were controlled by confocal microscopy using a fluorescent polyelectrolyte (rhodamine-PAH). The data suggest that decellularized cryopreserved arteries represent a potential scaffold for further vascular tissue engineering efforts. Moreover, the multilayer films can be used to coat biological surfaces and following the terminated layer (PAH or PSS), favour the cell adhesion or cell resistance.
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PMID:Decellularized umbilical artery treated with thin polyelectrolyte multilayer films: potential use in vascular engineering. 1682 3

Multilayer microcapsules showing unique charge-controlled permeability have been successfully fabricated by employing poly(styrene sulfonate) (PSS)-doped CaCO3 particles as templates. Encapsulation of the PSS molecules is thus achieved after core removal. Scanning force microscopy (SFM), UV-vis, Raman spectroscopy, and zeta-potential confirm the existence of the PSS molecules in the CaCO3 particles and the resultant microcapsules, which are initially incorporated during the core fabrication process. A part of these additionally introduced PSS molecules interacts with PAH molecules residing on the inner surface of the multilayer wall to form a stable complex, while the other part is intertwined in the capsule wall or in a free state. Capsules with this structure possess many special features, such as highly sensitive permeability tuned by probe charge and environmentally controlled gating. They can completely reject negatively charged probes, but attract positively charged species to form a higher concentration in the capsule interior, as evidenced by confocal microscopy. For example, the capsules completely exclude dextran labeled with fluorescein isothiocyanate (FITC-dextran), but are permeable for dextran labeled with tetramethylrhodamine isothiocyanate (TRITC-dextran) having similar molecular mass (from 4 to 70 kDa), although there are only few charged dyes in a dextran chain. By reversing the charge of the probes through pH change, or by suppressing charge repulsion through salt addition, the permeation can be readily switched for proteins such as albumin or small dyes such as fluorescein sodium salt.
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PMID:Charge-controlled permeability of polyelectrolyte microcapsules. 1685 39

A probe beam deflection (PBD) study of ion exchange between an electroactive polymer poly(allylamine)-bipyridyl-pyridine osmium complex film and liquid electrolyte is reported. The PBD measurements were made simultaneously to chronoamperometric oxidation-reduction cycles, to be able to detect kinetic effects in the ion exchange. Layer-by-layer (LbL) self-assembled redox polyelectrolyte films with osmium bipyridyl complex covalently attached to poly(allylamine) (PAH-Os) and poly(styrene sulfonate) (PSS) have been built by alternate electrostatic adsorption from soluble polyelectrolytes. The ionic exchange during initial conditioning of the film ("break-in") undergoing oxidation-reduction cycles and recovery after equilibration in the reduced state have shown an exchange of anions and cations with time lag between them. The effect of the nature of cation on the ionic exchange has been investigated with dilute HCl, LiCl, NaCl, and CsCl electrolytes. The ratio of anion to cation exchanged at the film-electrolyte interface has a strong dependence on the nature of charge in the topmost layer, that is, when negatively charged PSS is the capping layer, a larger proportion of cation exchange is observed. This demonstrates that the electrical potential distribution at the redox polyelectrolyte multilayer (PEM)/electrolyte interface determines the ionic flux in response to charge injection in the film.
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PMID:Dynamics of ion exchange between self-assembled redox polyelectrolyte multilayer modified electrode and liquid electrolyte. 1688 54

Adhesion of PAH/PSS and PDADMAC/PSS capsules through electrostatic and specific interactions has been investigated using reflective interference contrast microscopy (RICM). Adhesion of capsules via electrostatic interactions was found to be spontaneous and strong. Capsules functionalized with poly(l-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG) did not exhibit significant adhesion (as determined by the adhesion area) to streptavidin-coated substrates, whereas capsules functionalized with biotinylated PLL-g-PEG showed a significantly larger adhesion area. Using continuum mechanical models, the total adhesion energies for these cases were calculated and were found to correspond to several tens of individual biotin-streptavidin pairs. The application of specific interactions such as the biotin-streptavidin system for controlled capsule adhesion has been demonstrated in this study.
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PMID:Adhesion of polyelectrolyte microcapsules through biotin-streptavidin specific interaction. 1690 79


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