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Query: UMLS:C1832526 (PCC)
 5,967 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The structural gene for ferredoxin I, petF, from the cyanobacterium Anabaena sp. strain PCC 7120 has been isolated from a recombinant lambda library. Mixtures of tetradecanucleotides and heptadecanucleotides, each containing all possible DNA sequences corresponding to two separate regions of the ferredoxin amino acid sequence, were synthesized and used as hybridization probes to identify a genomic clone containing the coding sequence for the petF gene. The sequence of the entire petF coding region and portions of the 3'- and 5'-flanking regions was determined. The DNA sequence of petF suggests that, in contrast to the nucleus-encoded plant protein, cyanobacterial apoferredoxin is not synthesized as a higher-molecular-weight precursor. The Anabaena petF gene is a single-copy gene. During growth on complete medium it was transcribed into a monocistronic mRNA species of approximately 500 bases that initiated 100 base pairs upstream from the petF coding region.
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PMID:Isolation and sequence of the gene for ferredoxin I from the cyanobacterium Anabaena sp. strain PCC 7120. 243 Sep 49

Using degenerate oligodeoxyribonucleotide primers based on conserved regions of the cell-division protein FtsZ, a 220-bp fragment of DNA was amplified by the polymerase chain reaction from Anabaena PCC 7120 (Ana). This fragment, which showed significant homology with Escherichia coli ftsZ, was used as a probe to isolate a 15-kb genomic clone containing ftsZ from an Ana DNA library. Sequence analysis revealed an open reading frame (ORF) encoding a protein of 379 amino acids, with 49% identity with E. coli FtsZ. Upstream of Ana ftsZ is a small, unidentified ORF, transcribed in the same direction. An ORF lying downstream of the ftsZ coding region and transcribed in the opposite orientation, shows homology with bacterial glutathione synthetase-encoding genes. Single copies of ftsZ have been identified in Ana and two other cyanobacteria. Multiple transcripts hybridising to ftsZ were detected by Northern hybridisation.
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PMID:Cloning and sequence of ftsZ and flanking regions from the cyanobacterium Anabaena PCC 7120. 755 85

We isolated a 38 kDa ssDNA-binding protein from the unicellular cyanobacterium Synechococcus sp. strain PCC 6301 and determined its N-terminal amino acid sequence. A genomic clone encoding the 38 kDa protein was isolated by using a degenerate oligonucleotide probe based on the amino acid sequence. The nucleotide sequence and predicted amino acid sequence revealed that the 38 kDa protein is 306 amino acids long and homologous to the nuclear-encoded 370 amino acid chloroplast ribosomal protein CS1 of spinach (48% identity), therefore identifying it as ribosomal protein (r-protein) S1. Cyanobacterial and chloroplast S1 proteins differ in size from Escherichia coli r-protein S1 (557 amino acids). This provides an additional evidence that cyanobacteria are closely related to chloroplasts. The Synechococcus gene rps1 encoding S1 is located 1.1 kb downstream from psbB, which encodes the photosystem II P680 chlorophyll a apoprotein. An open reading frame encoding a potential protein of 168 amino acids is present between psbB and rps1 and its deduced amino acid sequence is similar to that of E. coli hypothetical 17.2 kDa protein. Northern blot analysis showed that rps1 is transcribed as a monocistronic mRNA.
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PMID:Structure and expression of the gene encoding ribosomal protein S1 from the cyanobacterium Synechococcus sp. strain PCC 6301: striking sequence similarity to the chloroplast ribosomal protein CS1. 786 84