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Query: UMLS:C1832526 (PCC)
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The cyanobacterium Synechocystis sp. strain PCC 6803 possesses two genes, named ppa and ppx, which, respectively, encode proteins involved in the hydrolysis of inorganic phosphate polymers, namely, inorganic pyrophosphatase (PPA, EC 3.6.1.1), an essential enzyme that hydrolyzes pyrophosphate, and exopolyphosphatase (PPX, EC 3.6.1.11), a processive enzyme that releases the terminal orthophosphate group from linear polyphosphates. Northern blots showed that both single-copy genes are induced by long-term inorganic phosphate (P(i)) starvation, transcript levels being markedly increased (ca. 10- and 20-fold, respectively) relative to P(i)-sufficient cells. Concurrent increases of both PPA and PPX specific activities and protein levels by P(i) deprivation were also observed. On the other hand, a knockout mutant was obtained by insertional mutagenesis of ppx, but it could not be achieved with ppa, thus indicating that PPA function is essential for cell viability. Moreover, whereas the ppx mutant exhibited under P(i)-sufficient conditions lower growth rates than the wild-type and was certainly devoid of PPX activity, it showed a severe reduction of the PPA levels. These results are the first evidence on the involvement of both PPA and PPX in a possible intracellular P(i)-recycling enzymatic process activated under P(i)-starvation.
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PMID:Concurrent transcriptional activation of ppa and ppx genes by phosphate deprivation in the cyanobacterium Synechocystis sp. strain PCC 6803. 1261 77

Soluble inorganic pyrophosphatases (inorganic diphosphatases, EC 3.6.1.1) were isolated and characterized from three phylogenetically diverse cyanobacteria--Synechocystis sp. PCC 6803, Anabaena sp. PCC 7120, and Pseudanabaena sp. PCC 6903--and one anoxygenic photosynthetic bacterium, Rhodopseudomonas viridis (purple nonsulfur). These enzymes were found to be family I soluble inorganic pyrophosphatases with c. 20 kDa subunits with diverse oligomeric structures. The corresponding ppa genes were cloned and functionally validated by heterologous expression. Cyanobacterial family I soluble inorganic pyrophosphatases were strictly Mg(2+)-dependent enzymes. However, diverse cation cofactor dependence was observed for enzymes from other groups of photosynthetic bacteria. Immunochemical studies with antibodies to cyanobacterial soluble inorganic pyrophosphatases showed crossreaction with orthologs of other main groups of phototrophic prokaryotes and suggested a close relationship with the enzyme of heliobacteria, the nearest photosynthetic relatives of cyanobacteria. A slow-growing Escherichia coli JP5 mutant strain, containing a very low level of soluble inorganic pyrophosphatase activity, was functionally complemented up to wild-type growth rates with ppa genes from diverse photosynthetic prokaryotes expressed under their own promoters. Overall, these results suggest that the bacterial family I soluble inorganic pyrophosphatases described here have retained functional similarities despite their genealogies and their adaptations to diverse metabolic scenarios.
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PMID:Comparative biochemical and functional studies of family I soluble inorganic pyrophosphatases from photosynthetic bacteria. 1763 82

Comparative proteomics together with physiological variables revealed different responses among three species of diazotrophic cyanobacterium Anabaena exposed to UV-B stress at the same time points. Perceptible decline in PSII activity, ATP pool, nitrogenase activity and respiration rate was observed for all the three species; this being maximum in Anabaena doliolum, followed by Anabaena sp. PCC 7120 and minimum in Anabaena L31. Statistical analysis of the protein abundance divided majority of them as early accumulated in A. L31, late accumulated in A. sp. PCC 7120 and downregulated in A. doliolum. Tolerance of A. L31 may be ascribed to post-translational modification reflected through the highest number of protein isoforms in its proteome followed by A. PCC 7120 and A. doliolum. Furthermore, increase in abundance of cyanophycinase, glutamine synthetase and succinate semialdehyde dehydrogenase in A. L31 suggests operation of an alternate pathway for assimilation of nitrogen and carbon under UV-B stress. An early accumulation of four proteins viz., glutamate ammonia ligase (Alr2328), transketolase (Alr3344), inorganic pyrophosphatase (All3570), and trigger protein (Alr3681) involved respectively in amino acid metabolism, energy metabolism, biosynthesis of cofactor and trigger protein and chaperone like activity across three species, suggests them to be marker of UV-B stress in Anabaena spp. This article is part of a Special Issue entitled: Proteomics in India.
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PMID:UV-B stress induced metabolic rearrangements explored with comparative proteomics in three Anabaena species. 2599 77