Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C1832526 (PCC)
 5,967 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The chloro-bridged dimer [(PC)Pt(mu-Cl)](2) (PC = [((t)Bu(2)P-o-C(6)H(4))-CMe(2)](-)) reacts with silver(I) acetate to yield the monomeric kappa(2)-acetate complex [(PC)Pt(kappa(2)-OOCMe)]. Its trifluoroacetate analogue exists in solution as a mixture of the monomer and two isomeric dimers, and crystallizes as the approximately C(2)-symmetrical dimer [(PC)Pt(mu-OOCCF(3))](2). NMR experiments and density functional theory calculations show that the monomeric species are favored by bulky ligands and electron-rich carboxylates. [(PC)Pt(OOCCF(3))] undergoes a ligand-dehydrogenation reaction in hot trifluoroacetic acid to yield [(PCC)Pt(OOCCF(3))(2)] (PCC = ((t)Bu(2)P-o-C(6)H(4))-C(Me)=CH(2)).
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PMID:Mononuclear platinum(II) complexes incorporating kappa2-carboxylate ligands: synthesis, structure, and reactivity. 1954 92

Microcystins are toxins produced by some strains of cyanobacteria. Several methods have been developed to allow the quantification of microcystins, which are mainly endotoxins. Among those methods, the protein phosphatase inhibition assay is a good candidate as a screening method because of its sensitivity, simplicity and specificity. In this work a method for intracellular microcystin extraction in field water samples and lab cyanobacterial cultures prior to their analysis by protein phosphatase inhibition assay has been optimized. Microcystin-LR and Microcystis aeruginosa PCC 7806 were used as reference microcystin and strain, respectively, in order to optimize the protocol. The protocol consists on filtering the sample through a nylon filter of 0.8 microm, filter extraction with methanol 80% 0.1% trifluoroacetic acid (TFA) 0.1% tween 20, extract centrifugation and supernatant dilution (1/20). The establishment of an extraction protocol was carried out determining the extraction volume, time of extraction and number of extractions. The advantages of the method developed in this work are basically its simplicity and avoiding the use of specific and expensive equipment.
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PMID:Optimization of intracellular microcystin-LR extraction for its analysis by protein phosphatase inhibition assay. 1980 54