Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C1832526 (
PCC
)
5,967
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Cyanobacterial 2-Cys peroxiredoxin (thioredoxin peroxidase,
TPX
) comprises a family of thiol antioxidant enzymes critically involved in cell survival under oxidative stress. In our previous study, a putative
TPX
was identified using a proteomics analysis of rice (
Oryza sativa
L.
japonica
,
OsTPX
) seedlings exposed to oxidative stress. This
OsTPX
gene is structurally similar to the
Synechococcus elongatus
TPX
gene in the highly conserved redox-active disulfide bridge (Cys114, Cys236) and other highly conserved regions. In the present study, the
OsTPX
gene was cloned into rice plants and
S. elongatus
PCC
7942 strain to study hydrogen peroxide (H
2
O
2
) stress responses. The
OsTPX
gene expression was confirmed using semi-quantitative RT-PCR and western blot analysis. The
OsTPX
gene expression increased growth under oxidative stress by decreasing reactive oxygen species and malondialdehyde level. Additionally, the
OsTPX
gene expression in
S. elongatus
PCC
7942 (OT) strain exhibited a reduced loss of chlorophyll and enhanced photosynthesis efficiency under H
2
O
2
stress, thereby increasing biomass yields twofold compared with that of the control wild type (WT) strain. Furthermore, redox balance, ion homeostasis, molecular chaperone, and photosynthetic systems showed upregulation of some genes in the OT strain than in the WT strain by RNA-Seq analysis. Thus,
OsTPX
gene expression enhances oxidative stress tolerance by increasing cell defense regulatory networks through the cellular redox homeostasis in the rice plants and
S. elongatus
PCC
7942.
...
PMID:Expression of
OsTPX
Gene Improves Cellular Redox Homeostasis and Photosynthesis Efficiency in
Synechococcus elongatus
PCC 7942. 3061 16
