Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C1522282 (EMT)
 2,868 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

At 43 degrees (but not at 41 degrees), the polyene antibiotic amphotericin B effectively inactivates mammalian cells in vitro even at doses which are used prophylactically, routinely, and continuously in some tissue culture laboratories. The greatly enhanced killing may reflect interactions between the drug and hyperthermia at the level of the cells' plasma membrane. A similar enhancement of cell killing at 43 degrees was seen when cells were exposed to nonisotonic salt solutions. Another polyene, nystatin, shows no temperature dependence, at least over the dose range examined, while another antifungal agent, polymyxin B, does so only at very high doses. The in vitro thermosensibility of cells to amphotericin B is reflected in vivo: EMT-6 murine tumor cells were killed much more efficiently in situ at 43 than at 37 degrees. Amphotericin B may be a useful agent in multiple drug thermochemotherapy.
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PMID:Interaction of amphotericin B and 43 degrees hyperthermia. 18 13

The effect of Amphotericin B (AMB) on radiogallium biodistribution in mice was studied at various time intervals. Two doses of AMB (1 mg/kg and 10 mg/kg) were administered intraperitoneally into mice bearing EMT-6 mammary sarcomas. The tumor and tissue uptakes of 67Ga were calculated when the radioactivity was administered either as 67Ga-citrate or 67Ga-transferrin (Ga-Tf). The role of transferrin was also investigated by using doubly-labeled 67Ga-125I-Tf. Our results indicate that both the doses of AMB and the chemical form of the radiogallium administered affect the pattern of distribution in some distinct fashion. It was also found that although the administration of AMB results in an increase in tumor-associated transferrin, this does not necessarily result in a concomitant increase in radiogallium uptake. In general, the effects of AMB on various tissues uptake of 67Ga appear to be related to both acute and subacute toxicity.
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PMID:The effect of amphotericin B on 67Ga biodistribution. 385 76