Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C1522282 (
EMT
)
2,868
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Background:
Circular RNA (circRNA) can regulate the progression of hepatocellular carcinoma (HCC). However, the role and potential mechanism of
circ_0004913
in HCC are not explored.
Methods:
Circ_0004913
was identified from two
GSE
datasets (GSE94508 and GSE97322) as a differentially expressed circRNA between HCC and normal tissues. Levels of
circ_0004913
, microRNA-184 (
miR-184
), and hepcidin (
HAMP
) were determined by quantitative real-time polymerase chain reaction (qRT-PCR). Cell proliferation, migration, and invasion were estimated by methyl thiazolyl tetrazolium, colony formation, and Transwell assays, respectively. Levels of all proteins were examined by Western blot. Glucose consumption and lactate and ATP production were analyzed by the glucose, lactate, and ATP assay kits. Dual-luciferase reporter, RNA immunoprecipitation (RIP), and RNA pull-down assays were performed to verify the interactions among
miR-184
and
circ_0004913
or
HAMP
. The mice xenograft models were established to assess the effect of
circ_0004913
on tumor growth
in vivo
.
Results:
Circ_0004913
was downregulated in HCC, and its expression impeded cell proliferation, migration, and invasion,
EMT
, and glycolysis in HCC cells.
miR-184
was identified as a target miRNA of
circ_0004913
, and their expression levels were negatively correlated.
miR-184
overexpression could reverse the inhibitory effect of
circ_0004913
on HCC cell progression. Moreover, as a target gene of
miR-184
,
HAMP
expression was positively correlated with
circ_0004913
expression in HCC tissues, and repression of
miR-184
could inhibit the progression of HCC cells by increasing
HAMP
expression.
Circ_0004913
could inhibit JAK2/STAT3/AKT signaling pathway and tumor growth
in vivo
by regulating the
miR-184
/
HAMP
axis.
Conclusion:
Circ_0004913
inhibited the tumorigenesis of HCC by sponging
miR-184
to regulate
HAMP
expression
in vitro
and
in vivo
.
...
PMID:
Circ_0004913
Inhibits Cell Growth, Metastasis, and Glycolysis by Absorbing
miR-184
to Regulate
HAMP
in Hepatocellular Carcinoma. 3302 99
