Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C1519176 (PSA)
 5,490 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Upon treatment with the phorbol ester, tetradecanoylphorbol 13-acetate (PMA), peripheral mononuclear blood cells from patients with acute myeloid leukemia secrete into serum-free cell-conditioned media (PMA-CCM) at least three distinct nondialysable 'hematopoietic' factors: granulocyte-colony-stimulating factor (G-CSF), granulocyte/macrophage-colony-stimulating factor (GM-CSF) and erythroid differentiation factor (EDF, activin A). G-CSF was identified by its stimulation of [3H]thymidine incorporation into a G-CSF-responsive cell line, NSF-60, and the inhibition of its stimulation by a G-CSF-specific monoclonal antibody (MAB). GM-CSF was identified by its stimulation of [3H]thymidine incorporation into a GM-CSF-responsive line, TALL-101, and the inhibition of its stimulation by a GM-CSF-specific MAB. EDF was identified by its ability to stimulate erythroid differentiation in mouse erythroleukemia cell lines, its identical retention times to those of authentic EDF on three successive reverse-phase HPLC columns and characterization of its penultimate N-terminal residue as leucine which is the same as that of authentic EDF. Both authentic EDF and the erythroid-stimulating activity in PMA-CCM were found to act synergistically with a suboptimal inducing concentration of a well-studied inducing agent, dimethyl sulfoxide, in inducing erythroid differentiation. In addition, a fourth activity was observed in PMA-CCM: normal human fetal bone marrow cell-proliferation stimulating activity (FBMC-PSA). FBMC-PSA was identified by its ability to stimulate the growth of granulocytes and macrophages in FBMC suspension cultures, which neither recombinant G-CSF or GM-CSF were found to do.
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PMID:Phorbol ester-treated human acute myeloid leukemia cells secrete G-CSF, GM-CSF and erythroid differentiation factor into serum-free media in primary culture. 170 23

A factor in mouse serum which stimulates proerythroblast proliferation in in vitro culture (proerythroblast stimulating activity-PSA) was purified by green A dye ligand and high performance liquid chromatography (HPLC). As judged by HPLC, PSA obtained after these two steps appeared to be a homogenous protein of Mr 100,000. It increased the proliferation of proerythroblasts when added alone to a liquid culture of bone marrow which was treated with anti-RBC antibody to remove haemoglobin containing erythroid cells. PSA functioned synergistically with erythropoietin (Ep) so that when added to culture together proliferation was 10-fold higher than when added to culture alone. PSA also increased CFU-E number but only when added together with Ep. Dose-response studies indicated that PSA increased CFU-E number when Ep remained constant and vice versa. PSA addition to culture could be delayed by as much as 12 h without any decrease in the number of CFU-E colonies that developed. When PSA was added to BFU-E cultures at the time of culture initiation no increase in BFU-E was seen. If, however, PSA was added 5 d after culture initiation BFU-E colonies were significantly increased. These results demonstrate that there is a factor (PSA) which can be purified from mouse serum which is not Ep. Its major site of action appears to be the more mature erythroid precursors that have the capacity to divide.
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PMID:Proerythroblast stimulating activity: its purification from mouse serum and its effect on mouse erythroid cell proliferation in vitro. 339 Mar 91