Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C1519176 (PSA)
 5,490 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Two fragments were isolated from BSA one was derived from the first terminal third of the molecule and the second from the last third of the molecule. Each fragment inhibited the reaction of BSA-anti BSA by 90% or better. An immunoabsorbent of each bound 90% of anti BSA. Each fragment bound two antibody molecules per mole of fragment. These results are explained by the concept that BSA contains repeating identical or similar antigenic determinants. Conformational non identity of various batches of BSA was revealed by reactivity of the disulfide bonds at the neutral transition. Trypsin was found to cleave GSA, PSA, and HSA to yield an immunochemically reactive fragment. At least in the case of HSA, the fragment exhibited all the immunochemical reactivity of the native protein.
 ...
PMID:Immunochemistry of bovine serum albumin. 8 78

The expression of a high molecular weight cell surface glycoprotein (LETS, fibronectin) by preimplantation mouse embryos as well as cultured teratocarcinoma stem cells was detected by using indirect immunofluorescent staining. When each stage of preimplantation embryonic development was tested for the presence of LETS protein, none was observed on two-cell, four-cell, or eight-cell embryos, or on the morula or the outer cell layer (trophectoderm) of the early or late blastocyst. However, when the inner cell mass was isolated by immunosurgery, positive staining was observed. The intensity of the staining was significantly greater on the inner cell mass isolated from the expanded (day 4) blastocyst than on that from the early (day 3) blastocyst. Certain established cell lines of teratocarcinoma stem cells (embryonal carcinoma cells) also express cell surface LETS protein. "Nullipotent" (Nulli-SCC-1) as well as pluripotent (PSA 1) embryonal carcinoma cell lines have deposits of LETS protein concentrated in areas of cell-cell contact. In addition, a teratocarcinoma-derived endodermal cell line (PYS) was found to be capable of depositing LETS onto the substratum in a fibrillar network.Taken together, our results indicate that LETS protein is synthesized at a specific stage of preimplantation mouse embryonic development. In particular, they suggest that LETS protein is a product of the embryonic ectoderm, and that some types of embryonic endoderm are also capable of synthesizing this protein.
 ...
PMID:Expression of a high molecular weight cell surface glycoprotein (LETS protein) by preimplantation mouse embryos and teratocarcinoma stem cells. 27 75

Cells from mice inoculated with picryl sulphonic acid (PSA cells), which contain suppressor T cells for contact sensitivity to picryl chloride, were examined for their ability to alter antibody responses of normal mice. These cells did not influence antibody or plaque-forming cell (PFC) production accompanying contact sensitivity reactions produced by painting with picryl chloride but reduced IgG antibody and indirect PFC responses to conjugates of trinitrophenyl (TNP) bovine serum albumin and ovalbumin. IgG responses to TNP or new antigenic determinants of TNP-mouse serum albumin were not affected by PSA cells. The PSA cells required several weeks to produce reductions of responses and only reduced responses to optimal doses of antigen. When the injection of antigen was delayed until several weeks after the injection of PSA cells rapid reductions of responses were found but these were short-lived. The inhibition was specific for TNP proteins although responses to hapten and carrier were reduced. Evidence is presented to show that the inhibition was mediated by an adherent macrophage-like cell rather than a T cell. The inhibitory activity was resistant to irradiation and anti-theta treatment but was removed by glutaraldehyde treatment and cotton wool filtration.
 ...
PMID:Inhibition of antibody responses by cells from mice treated with picryl sulphonic acid. 32 2

Sera obtained from planned immunizations between unrelated donors and recipients, identical or compatible at HLA-A and B, were assessed for their capacity to alter the in vitro response of a test panel of lymphocytes to PHA and a purified streptococcal antigen (PAS). In the case of PHA, no serum effects were apparent. The response to PAS, however, significantly inhibited by two sera. When tested for their complement-dependent cytotoxicity on enriched populations of T and B lymphocytes, none of the sera manifested cytotoxicity against T cells nor did serological inhibition correlate with the capacity to lyze B cells. The data suggest that inhibition of the PSA response is mediated by blocking antibodies specific for a subset of lymphocytes, possibly T cells. While the precise mechanism governing the response to PSA is not known, the data are compatible with the idea that an HLA-linked Ir gene, expressed on a subset of T lymphocytes, controls immune responsiveness to PSA.
 ...
PMID:Serological inhibition of blast transformation to purified streptococcal antigens by planned immunization in HLA (A,B) compatible unrelated individuals. 41 52

The authors examined the frequency of cells with polyploidy-P, with amitot division-AD and with mitotic division-MD in 25 patients with schizophrenia and proven anticerebral autoimmune sensibilization by means of blast-transformation test-BS, in 20 partners of marriages with multiple spontaneous abortions and suspected isoimmunization-PCA as well as control group of 63 healthy adults and newborn infants. Simultaneous increase of the frequency of cells with polyploidy and amitotic division was established in patients with schizophrenia--1,16% of P and 0.40% AD in 4 women with spontaneous abortiosn -- 1.5% of P and 0.80% of AD compared with the control values of 0.54% of P and 0.17% of AD. The highest percentage P -- 1.12 and AD -- 2.1 was found in BS with chronic process, in whom the blast-transformation reaction to brain antigen --34% was mostly manifested. In the cultures of BS, treated with brain antigen, AD reached up to 1.38%. The increase of AD was accompanied with lowering of mitotic index: BS -- 1.2% of MD and 0.40 of AD; PSA -- 2,04% of MD and 0.50 of AD compared with the control values of 4% of MD and 0.17% of AD. The results showed that immunologic sensibilization was accompanied by an increase of genome mutations and amitotic division and with lowering of mitotic index; they revealed polyploidy and amitotic division as compensatory mechanisms of the organisms under certain conditions.
 ...
PMID:[Correlation between genome mutations and the cell division types in immunological sensitization states]. 49 37

An attempt was made to correlate serological relationships determined by the pili, the flagella and the O-somatic antigens of Pseudomonas aeruginosa, and to make a preliminary assessment of use of the pilus antigen as an epidemiological marker. A method is described for the preparation of antiserum specific for the "normal' PSA pili of P. aeruginosa. A high titre of pilus antibodies was obtained by immunizing rabbits with mutants whose pili had lost their ability to retract into the cell. The "normal' form of the organism, with retractile pili, was poorly agglutinated by high-titre anti-pilus serum, but suspensions of it that had been treated with osmium tetroxide showed greatly increased agglutinability. Antibody labelling for electron microscopy was used to determine the serological relations of pili and of flagella for P. aeruginosa strains belonging to different serological groups as defined by O-somatic antigens. The distribution of pilar and flagellar antigens among strains was not correlated with the O-somatic serotype. A strain of P. aeruginosa carrying a drug-resistance plasmid had fewer "normal' PSA pili than the background strain.
 ...
PMID:An immunological study of the pili of Pseudomonas aeruginosa. 80 17

Results of surgical treatment of prehepatic portal hypertension during the past 15 years were assessed, in addition to routine clinical and laboratory studies, by splenoportography and control mesenteric-venography. One patient survived 14 years. Control angiography of the portal system proved to be most important in assessing the results of surgical treatment, recognizing the cause of late failures and evaluating the course of the disease. All splenectomized patients with general PPH experienced recurrences of hemorrhage which required reoperation. Direct operation on the varices is a favorable procedure, particularly when done as an emergency procedure. Following surgery, early angiographic examination, especially by mesenteric-venography, is mandatory. In cases with poor portal circulation through the liver and massive passage of blood through the varices, PSA should be performed without delay. Portosystemic anastomosis is a selective procedure. Without angiographic study it is contraindicated as an emergency procedure, especially in splenectomized patients.
 ...
PMID:Surgery and prognosis of prehepatic portal hypertension. 116 77

A human primary prostatic adenocarcinoma cell line named ND-1 has been established in long term tissue culture. The cultured cells show growth in both monolayers and in soft agar suspension and produce subcutaneous tumors in nude mice. Cytogenetic analysis by G-banding demonstrated an aneuploid karyotype with a modal chromosome number of 62, and multiple marker chromosomes with 25-30% structural abnormalities. Ploidy analysis revealed that the majority of ND-1 cells (67%) had a DNA mass of 10.1 picogram and DNA index of 1.41. Nineteen percent of cells had a DNA mass of 21.3 picogram and DNA index of 3.0. Electron microscopic studies revealed common features of neoplastic epithelial cells such as numerous microvilli, junctional complexes, abnormal nuclei, nucleoli, and mitochondria. Due to the absence of a structurally normal Y chromosome, confirmation of the presence of a derived Y chromosome was achieved through the use of C-banding and through fluorescent in situ hybridization with a Y chromosome repeat probe. Tandem E PSA immunoenzymatic assay revealed that these ND-1 cells secrete small amounts of prostate specific antigen.
 ...
PMID:Establishment and characterization of a human primary prostatic adenocarcinoma cell line (ND-1). 127 21

It is well established that single lead VDD pacing is a physiological, reliable, and easy to use mode of pacing. The major limitation of VDD pacing is the need of a normal sinus node function, confirming its indication to patients with isolated atrioventricular conduction disturbances. The Phymos 830 pacing lead was originally designed for VDD pacing in association with the Phymos MPS pulse generator; it has a floating atrial dipole with an interelectrode distance of 3 cm; the distal electrode is 11, 13, or 15 cm proximal to the tip. As a result of the incidental observation of atrial captures occurring at very low pulse amplitudes delivered from the floating dipole of this lead, a 13-center Italian study was initiated to test the systematic feasibility of this type of atrial pacing. Pacing parameters were set and strength-duration curves were acquired with the PSA Master 470 external device. The investigation was performed at pacemaker implant in 114 patients in the supine position. The tip of the electrode was positioned at the right ventricular apex and the atrial dipole at the site of the highest endocavitary signal. Atrial bipolar pacing was performed with the proximal electrode as the cathode. Stable atrial capture was obtained in 108 of 114 patients (94.7%); pacing threshold was < 3.5 V with a pulse width of 1 msec in 85 of 108 patients. Results of voltage threshold were: 2.99 +/- 1.25 and 2.59 +/- 1.13 V at pulse widths of 0.5 and 1 msec, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
 ...
PMID:Atrial stimulation by means of floating electrodes: a multicenter experience. The Multicenter Study Group. 127 83

In view of the limitations associated with the present tumor markers for prostate cancer, we have examined the potential expression of two further markers, Cathepsin-D and pS2, in human prostate and attempted to link their concentrations with the histopathology of the tissue, the PSA levels and the androgenic status of the gland. Cathepsin-D and pS2 were measured in cytosol fractions obtained from 22 patients with benign prostatic hyperplasia (BPH) and 20 patients with prostate cancer (CaP) employing immunoassays specific for these markers. The concentrations of Cathepsin-D (BPH: mean +/- SEM = 18.50 +/- 1.88 nmol/g protein; CaP = 19.75 +/- 2.49 nmol/g protein) and pS2 (BPH = 1,024.7 +/- 348.06 ng/g protein; CaP = 1,513.88 +/- 268.60 ng/g protein) were not different in the two tissue types, whereas PSA in BPH tissue (1,952.27 +/- 249.93 micrograms/g protein) was significantly higher than the measurements in CaP (583.75 +/- 104.33 micrograms/g protein). However, none of the tumor marker concentrations correlated with the degree of differentiation of the tumors, and we were unable to establish any correlation with the levels of testosterone and dihydrotestosterone in the tissue. In conclusion, although Cathepsin-D and pS2 are expressed in prostate tissue, it is doubtful whether they will have an active role in the management of prostate cancer.
 ...
PMID:The distribution of PSA, cathepsin-D, and pS2 in BPH and cancer of the prostate. 127 46


1 2 3 4 5 6 7 8 9 10 Next >>