Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C1389183 (autodigestion)
 317 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Capillary electrophoresis (CE) and matrix assisted laser desorption time-of-flight mass spectrometry (MALDI-TOF-MS) were investigated as alternatives to sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis for peptide mapping with Staphylococcus aureus protease (V8) of a hydrophobic recombinant hepatitis C virus antigen, HC-31, which required 0.1% SDS for solubility. Controls (V8 only) or HC-31 digests were extracted with chloroform-methanol-water (1:4:3) to remove SDS, which interferes with MALDI-TOF, and high salt content, which affects CE. In two different runs by CE, the elution times of each of 11 peptide peaks were very reproducible (R.S.D. < 0.016). 25 fragments were resolved by MALDI-TOF-MS, including six smaller peptides (M(r) < 13 000) resulting from V8 autodigestion. MALDI-TOF-MS indicated that partial cleavages occurred, primarily at sites where there are paired glutamic and/or aspartic acid residues.
 ...
PMID:Comparative peptide mapping of a hepatitis C viral recombinant protein by capillary electrophoresis and matrix-assisted laser desorption time-of-flight mass spectrometry. 884 66

1. By means of certain chemical reagents, normal guinea pig serum can be brought to autodigestion without the presence of any foreign substrate. There exists in normal sera a highly characteristic protease. 2. The serum ferment survives heating at 55 degrees C. for 30 minutes, but is completely inactivated at 60 degrees C. for the same length of time. 3. The autodigestion of serum requires a temperature of about 37 degrees C., and no noticeable digestion takes place at a temperature of 16 degrees C. or lower. 4. Autodigestion of the serum may be brought about by chloroform and various saturated monovalent ketones and alcohols of the lower series. 5. The ketones and alcohols have a certain narrow limit of concentration for activating serum, beyond which the ferment is destroyed, even at room temperature. 6. The ketones and alcohols in concentrations regulated to activate serum at room temperature destroy the ferment when allowed to act on serum at 37 degrees C. for 30 minutes. The elimination of the concentrated reagents from serum by evaporation or dialysis protects the ferment from their destructive action. 7. A certain length of time is required for the chemical activators to complete their action. In this respect chloroform is much slower than acetone. 8. The chemical activators may be removed from the activated serum by means of vacuum, dialysis, or extraction with certain indifferent chemicals without causing a return of the serum to its original non-autolytic state. Once activated by these reagents, the serum remains in the activated state, in spite of the removal of the activators. 9. The ferment is highly sensitive to the reaction of the medium, being readily inactivated when the reaction exceeds a certain narrow limit towards acid or alkaline. The optimal digestion is obtained with a faintly alkaline or neutral reaction.
 ...
PMID:THE AUTODIGESTION OF NORMAL SERUM THROUGH THE ACTION OF CERTAIN CHEMICAL AGENTS. I. 1986 35