Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C1275122 (TEM)
 21,810 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The ability to form spontaneous E (rabbit erythrocyte; RRBC) rosettes has been established as the characteristics common to T lineage lymphocytes in the guinea pig as in the case of human (sheep erythrocyte; SRBC), and the E rosette method has been widely employed to identify and to purify T cells. Since the author was interested whether the T cells purified by this method could be regarded as the physiologically normal T cells, several experiments were carried out followed by the results as listed below. (1) Significant increases in percentages of EA- and EAC-rosette forming cells (RFC) were observed among guinea pig thymocytes and lymph node cells following the formation of spontaneous E rosettes with RRBC in test tubes. (2) Similar but somewhat higher increases in the proportion of EA- and EAC-RFC were observed in the two T cell-rich populations, one was T-enriched by the E monolayer method and the other was done by the nylon wool column method and the E monolayer method. In the both cell populations, the E monolayer-adhered cells (T cells) were selected and assayed on the E monolayers. (3) Double rosette assays by E with EA or EAC showed that 50-80% of the Fc and/or complement receptor positive lymphocytes on the E monolayers were E-RFC. These findings show that a subset of the guinea pig T cells is altered to express Fc and/or complement receptors on the surfaces following contact with RRBC.
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PMID:[Changes of guinea pig T lymphocytes after E rosette formation -expression of Fc and complement receptors-(author's transl)]. 697 98

Mononuclear cells from 10 healthy blood donors were incubated with ethanol at 37 degrees C for 30 min. An ethanol concentration of 10.0 g/l reduced the percentage of active E rosette-forming cells (E RFC) from 25.0 +/- 12.1 to 16.2 +/- 8.7, and the percentage of total E RFC from 70.3 +/- 8.5 to 59.7 +/- 13.5 (p less than 0.01 for both). The percentage of cells with phagocytizing capacity was reduced from 11.7 +/- 5.4 to 7.0 +/- 5.0 by the ethanol treatment (p less than 0.01). Incubation with ethanol at a concentration of 1.0 g/l also significantly reduced the number of active and total E RFC and of phagocytizing cells. Ethanol at a concentration of 0.1 g/l did not influence these cell subpopulations. The number of EA RFC and EAC RFC were not influenced by ethanol.
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PMID:In vitro effect of ethanol on subpopulations of human blood mononuclear cells. 698 Jan 94

Lymphocytes from heparinized or defibrinated blood were separated on Lymphoprep and washed in phosphate-buffered saline (PBS) or Hanks' balanced salt solution (HBSS). Defibrination caused a decreased yield of lymphocytes compared to heparin treatment. The cell loss was probably non-selective, as only minor differences in lymphocyte subpopulations were found. However, lymphocytes from defibrinated blood, washed in PBS gave a lower percentage of rosette-forming cells (RFC) in most tests, and higher number of latex-phagocytizing cells. For the stabilization of sheep erythrocyte (E)-RFC, treatment of E with 2-amino-ethylisothiouronium bromide hydrobromide (AET), with addition of fetal calf serum (FCS), and E-RFC without FCS fixed with glutaraldehyde gave similar results, and higher percentages of RFC than the RFC test performed with FCS. Storage of whole blood or separated lymphocytes for 24 h at 4 degrees C generally resulted in a reduction in the percentages of E-RFC, particularly active E-RFC, but not of EA- or EAC-RFC. The ranges of the results were usually wider after storage.
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PMID:Technical aspects of rosette tests for the demonstration of lymphocyte subpopulations. 708 Aug 38

A subpopulation of adenoidal lymphocytes was determined by the E- and EAC-rosetting techniques in order to study an immunological profile of adenoids in 61 children with recurrent otitis media, rhinosinusitis or recurrent tonsillitis. Though there was no significant difference in E- and EAC-rosette forming cells of adenoid tissues from children with recurrent infection in the upper respiratory tract, our results indicated the following. (1) A higher proportion of EAC-rosette forming cells (EAC-RFC) without a change of E-RFC was found in the adenoids of children with recurrent tonsillitis than those without it. (2) The percentage of EAC-RFC appears to increase proportionally to the size of adenoid viewed on the X-ray film. (3) The higher percentage was more remarkable in cases with rhinosinusitis and recurrent otitis media. From the data obtained it is concluded that adenoids may play some part in immunity responses against infection in the upper respiratory tract reflecting adenoidal hypertrophy.
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PMID:Subpopulation of adenoidal lymphocytes of recurrent infection in the upper respiratory tract. 718 59

Lymphocytes from 34 patients with active and with stable multiple sclerosis (MS) were examined by rosetting techniques. Patients with active disease had a relative lymphopenia (35 +/- 9%). When tested with erythrocytes (E) from one out of three sheep used, patients with active MS had a decrease in E-rosette forming cells (E-RFC) compared to patients with stable disease and with healthy controls. Other tests (active E-, EAET-, EA-, and EAC-RFC) did not disclose any differences between patients and controls. The results suggest that the origin of the E may be of importance in E-RFC tests.
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PMID:Lymphocyte subpopulations in multiple sclerosis: variations in rosette tests using erythrocytes from different sheep. 724 44

A mutant Chinese hamster ovary cell line, glyB, that required exogenous glycine for survival and growth was reported previously (Kao, F., Chasin, L., and Puck, T. T. (1969) Proc. Natl. Acad. Sci. U. S. A. 64, 1284-1291). We now report that the defect in glyB cells causative of this phenotype is a point mutation in an inner mitochondrial membrane protein required for transport of folates into mitochondria. The CHO mitochondrial folate transporter (mft) was sequenced and compared with that from glyB cells. The hamster sequence was nearly identical to that of the recently reported human mitochondrial folate transporter. The corresponding cDNA from glyB cells contained a single nucleotide change that introduced a glutamate in place of the glycine in wild-type hamster MFT at codon 192 in a predicted transmembrane domain. Transfection of the wild-type hamster cDNA into glyB cells allowed cell survival in the absence of glycine and the accumulation of folates in mitochondria, whereas transfection of the Glu-192 cDNA did not. Genomic sequence analysis and fluorescence in situ hybridization demonstrated a single mutated allele of the mft gene in glyB cells, whereas there were two alleles in CHO cells. We conclude that we have defined the cause of the glyB auxotrophy and that the glyB mft mutation identified a region of this mitochondrial folate carrier vital to its transport function.
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PMID:A mutation inactivating the mitochondrial inner membrane folate transporter creates a glycine requirement for survival of chinese hamster cells. 1514 Aug 90


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